PLGA缓释微球的制备及其释药降解性能研究

The preparetion of PLGA microspheres and the research about the capability of the control release

以巴比妥为球心物质,聚乙烯吡咯烷酮(PVP)为分散剂,采用溶剂挥发法制备了聚(乳酸-羟基乙酸)共聚物PLGA载药微球。透射电镜、光学显微镜测试表明微球球型规则,表面平滑,分布均匀,微球粒径在400nm左右,包覆效果良好,微球载药率1.039%,药物包封率42.34%。红外(FT-IR)分析得知,两种物质互相融为一体。以PH=7.4的PBS缓冲溶液为释放介质,用紫外分光光度计(UV)对微球的体外释药过程进行了实验,微球在前10天有明显的突释,此后缓慢释药,45天后药物释药率在80%以上。实验结果表明:PLGA是一种理想的控缓释材料。

The aim of this study was first to prepare Poly （lactide-co-glycolide） PLGA microsphere containing barbitone by a solvent evaporation method,PVP was used as dispersant . Transmission Electron Microscope （TEM） and Optical Microscope （OM） showed that the preparation of microsphere had regular shape , smoother surfaces and and relatively narrow particle size distribution. Particle size is 400nm, approximately. The embedding effect was preferable. The actual drug loading was 1.039% and Encapsu!ation efficiency was 42.34%. According to Fourier transform infrared spectroscopy （FT-IR） analysis, PLGA and barbitone had become one substance. PH=7.4 PBS buffer solution was used as releasing media. UV was used to analyse the process of the release of microsphere, In the first 10 days, an initial burst release was observed . subsequently, drug release followed by a nearly constant rate per day. The cumulative amount of drug release was exceeded 80% after 45 days. The result showed that PLGA was a appropriate material of control release.