HBeAg阴性乙肝患者LHBs的检测及其意义

Clinical Significance of Measuring Hepatitis B Virus Large Surface Protein in Patients with HBeAg-negative Chronic Hepatitis B Blood

目的探讨检测乙型肝炎HBeAg阴性患者病毒表面大蛋白(LHBs)用于反映体内乙型肝炎病毒复制的意义。方法采用酶联免疫吸附实验(ELISA)和荧光定量PCR法对807例HBeAg阴性血清标本进行LHBs及HBV DNA检测。结果1在807例HBeAg阴性慢性乙肝血清中HBV DNA阳性率为54.40(439/807),LHBs的阳性率为56.75(458/807),二者比较无显著性差异(χ2=0.813,P>0.05),两者总体符合率为91.70(740/807);2HBeAg阴性慢性乙肝不同HBV-M模式的HBV-DNA与LHBs检出结果均无显著性差异(P>0.05)。3LHBs含量与HBV DNA拷贝数呈正相关(r=0.995,P<0.001)。结论LHBs与HBV DNA具有良好的相关关系,能够反映出HBeAg阴性乙肝患者体内HBV复制的程度,是HBV DNA复制水平判定的有效指标,具有重要的临床意义。

Objective To explore the significance of hepatitis P, virus large surface protein（LHBs） in the diagnosis of viral repication in patients with HBeAg-negative chronic hepatitis B. Methods Enzyme linked immunosorbent assay（ELISA） methods were used to examine the LHBs and quantitative real-time PCR methods were used to detect the HBV DNA of 807 HBeAg-negative chronic hepatitis B serum samples. Results （1）No significant difference of positive rate was observed between HBV DNA 54.40%（439/807） and LHBs 56.75%（458/807）（Χ^2=0. 813,P〉0. 05） in 807 HBeAg-negative chronic hepatitis B serum samples,the collectivity accord rate was 91.70% （740/807） between LHBs and HBV-DNA. （2）The expression of HBV-DNA and LHBs were not significantly different among various patterns of HBV serological marks either （P〉0. 05）. （3）Serum LHBs levels were correlated with the serum HBV DNA copies （r= 0. 995,P〈0. 001 ）. Conclusion The results demonstrated that measurement the quantity of serum LHBs might helpful to estimate the state of HBV DNA replication.