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致倦库蚊Cecropin基因克隆及序列分析 被引量：2

Cloning and Sequence Analysis of A Cecropin Gene of Culex quinquefasciatus
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摘要 目的:克隆致倦库蚊Cecropins基因序列,并进行生物信息学分析。方法:选取致倦库蚊成虫,提取其DNA并以此为模板,设计一对引物,采用PCR技术扩增出Cecropins基因,应用相关生物信息学软件分析其序列。结果:克隆出致倦库蚊Cecropins基因,并测定其扩增产物序列,经同源对比确认,进行了相关序列分析。结论:获得致倦库蚊Cecropins基因序列,基因长度为259bp,与尖音库蚊一致性为97%。 Objective： To clone and analyze the sequence of cecropin gene of Culex quinquefasciatus. Methods ： Genomic DNA of C. quinquefasciatus adults was isolated, and was used as template to clone cecropin gene by PCR with a pair of designed primers ATGAACTrCAACAAGCTTF and CAAGTC-CTACT TYCCAAGT. The obtained sequence was analyzed with bioinformatics softwares. Results： A cecropin gene of C. quinquefasciatus was obtained, and was sequenced and analyzed. The results showed that the gene was 259 bp and was 97% consistent with that of cecropin gene of C. pipiens. Conclusion： The cloning is successful, and one of the cecropin genes of C. quinquefasciatus was obtained.
作者 康哲 张春林 陈汉彬
机构地区 贵阳医学院生物学教研室
出处 《贵阳医学院学报》 CAS 2008年第6期643-646,651,共5页 Journal of Guiyang Medical College
基金 贵州省科学技术基金资助项目[黔科合丁字(2006)2077号]
关键词 库蚊属 基因cecropin 基因顺序 Culex gene cecropin gene order
分类号 R696.442 [医药卫生—泌尿科学]
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参考文献7

  • 1Boman HG, Ingrid Nilsson Faye, Kerstin Paul, et al . Charcteristics of an inducible cell-free antibacterial reation in hemolymph of samia Cynthia pupae [ J ]. Infect hnmun, 1974(10) :136 - 145.
  • 2Kokoza V, Ahmed A ,Cho W L, et al. Engineering blood mealactivated systiced syste mic immunity in the yellow fever mosquito , aedes aegypti [ J ]. Pro Natl Acad Sci USA,2000 (16) :9144 -9149.
  • 3支国舟,陈晓光,周晓红,彭鸿娟,林立丰,易建荣,江静娴,张淑萍.蚊防御素基因的克隆及序列分析[J].第一军医大学学报,2002,22(6):499-502. 被引量：5
  • 4刘先凯,赵彤言,朱礼华,董言德,陆宝麟.白纹伊蚊和埃及伊蚊防御素(defensin)成熟肽基因克隆及结构推测[J].寄生虫与医学昆虫学报,2003,10(1):30-34. 被引量：5
  • 5Lowenberger C, Charlet M, Vizioli J, et al. Antimicrobial activity spectrum, cDNA cloning, and mRNA expression of newly isolated member of the cecropin family from the mosquito vector Aedes aegypti [ J ]. Biol Chem, 1999 (274) : 20092 - 20097.
  • 6Lyric C Bartholomay , Hoda A Farid , Reda M Ramzy, et al. Culex pipiens pipiens: characterization of immune peptides and the influence of immune activation on development of Wuchereria bancrofti [ J ]. Molecular & Biochemical Parasitology, 2003 (130) :43 - 50.
  • 7Ravi K Rauniyar, Kiyoshi Suzuma, Adam L King, et al. Differential effects of bactericidal permeability-increasing protein (BPI) analogues on retinal neov-ascularization and retinal pericyte growth [ J ]. Investigative Ophthalmology & VisualScience ,2002 (2) :503 - 509.

二级参考文献9

  • 1[7]Eggleston P, Lu W, Zhao Y. Genomic organization and immune regulation of the defensin gene from the mosquito [J]. Jnsect Mol Biol, 2000, 9(5): 481-90.
  • 2[8]Cho WL, Fu TF, Chiou JY, et al. Molecular characterization of a defensin gene from the mosquito [J]. Insect Biochern Mol Biol,1997, 27(5): 351-8.
  • 3[2]Vernick KD. Mechanisms of immunity and refractoriness in insect vectors of eukaryotic parasites [A]. In: Molecular mechanisms of immune responses in insects[M]. London: Chapman & Hall, 1998.261-309.
  • 4[3]Matsuyama K, Natori S. Purification of three anti-bacterial proteins from the culture medim of NIH-Sape-4, an embryonic cell line of Sareophagaperegrina[J]. J Biol Chem, 1988, 263(30): 17112-6.
  • 5[4]Lambert J, Keppi E, Dimarcq JL, et al. Insect imunity: isolation from immune blood of the dipteran phormia terrsnovae of two insect anti-bacterial peptides with sequence homology to rabbit lung macrophage bactericidal peptides [J]. Proc Natl Acad Sci USA,1998, 86(1): 262-6.
  • 6[5]Dimarcq JL, Hoffmann D, Meister M, et al. Ch3racterization and transcriptional profiles of a drosophila gene encoding an insect defensin. A study in insect defensin [J]. Eur J Biochem, 1994, 221(1): 201-9.
  • 7[6]Chalk R, Townson H, Natori S, et al. Purification ofan insect defensin from the mosquito aedes aegypti [J]. Insect Biochem Mol Biol, 1994, 24(4): 403-10.
  • 8[1]Collins FH, Sakai RK, Vemick KD, et al. Genetic selection of a Plasmodium-refractory strain of the malaria vector Anopheles gambiae[J]. Science, 1986, 234: 607-9.
  • 9Jean-Marc Bonmatin,Jean-Luc Bonnat,Xavier Gallet,Fran?oise Vovelle,Marius Ptak,Jean-Marc Reichhart,Jules A. Hoffmann,Elisabeth Keppi,Michèle Legrain,Tilman Achstetter. Two-dimensional1H NMR study of recombinant insect defensin A in water: Resonance assignments, secondary structure and global folding[J] 1992,Journal of Biomolecular NMR(3):235～256

共引文献5

同被引文献24

  • 1磨健悦,孙宝丽,魏钟艳,曾喜多,曾志良,谢青梅,毕英佐,马静云.猪流行性腹泻病毒COE基因的原核表达及其表达产物的生物活性分析[J].中国兽医科学,2015,45(5):503-508. 被引量：2
  • 2Boman H.G., Nilsson I., and Rasmuson B., 1972, Inducible antibacterial defence system in drosophila, Nature, 237(5352): 232-235.
  • 3Cheng T., Zhao P., Liu C., Xu P., Gao Z., Xia Q., and Xiang Z., 2006, Structures, regulatory regions, and inductive expression patterns of antimierobial peptide genes in the silkworm Bombyx mori., Genomics, 87(3): 356-365.
  • 4Godreuil S., Leban N., Padilla A., Hamel R., Luplertlop N., Chauffour A., Vittecoq M., Hoh F., Thomas F., Sougakoff W., Lionne C., Yssel H., and Misse D., 2014, Aedesin: structure and antimicrobial activity against multidrug resistant bacterial strains, Plos One. 9(8): e105441.
  • 5Hong S.M., Kusakabe T., Lee J.M., Tatsuke T., Kawaquchi Y., Kang M.W., Kang S.W., Kim K.A., and Nho S.K., 2008, Structure and expression analysis of the cecropin-E gene from the silkworm, Bombyx mori, Biosci. Biotechnol. Biochem., 72(8): 1992-1998.
  • 6Hultmark D., Engstrom A., Bennich H., Kapur R., and Boman H. G., 1982, Insect immunity: isolation and structure ofcecropin D and four minor antimicrobial components from Cecropia pupae, European Journal of Biochemistry, 127(1): 207-217.
  • 7Kato Y., Taniai K., Hirochika H., and Yamakawa M., 1993, Expression and characterization of cDNAs for Cecropin B, an antibacterial protein of the silkworm, Bombyx mori., Insect Biochem. Mol. Biol., 23(2): 285-290.
  • 8Lowenberger C., Charlet M., Vizioli J., Kamal S., Richman A., Christensen B.M., and Bulet P., 1999, Antimicrobial activity spectrum, cDNA cloning, and mRNA expression of a newly isolated member of the cecropin family from the mosquito vector Aedes aegypti, Journal of Biological, 274 (29): 20092-20097.
  • 9Magalhaes T., Oliveira I.F., Melo-Santos M.A.V., Oliveira C.M.F., Lima C.A., and Ayres C.F.J., 2008, Expression of defensin, cecropin, and transferrin in Aecles aegypti (Diptera: Culicidae) infected with 1Vuchereria bancrofti (Spirurida: Onchocercidae), and the abnormal development of nematodes in the mosquito, Experimental Parasitology, 120(4): 364-371.
  • 10Ponnuvel K.M., Natarajan S., Sasibhushan S., Murthy G.N., and Vijayaprakash N.B., 2010, Molecular evolution of the cecropin multigene family in silkworm Bombyx mori, Bioinformation, 5(3): 97-103.

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贵阳医学院学报
2008年 第6期

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