摘要
利用测序技术和生物信息学分析技术,对关中马27个个体的线粒体DNA D-loop 247 bp序列的遗传多态性及系统进化进行分析,检测到9种单倍型,其核苷酸多态位点19个,约占所测核苷酸总长的7.69%,均为转换。关中马mtDNA D-loop区核苷酸多样度(π值)为0.0219±0.0076,单倍型多样度(H)为0.843±0.041,表明关中马mtDNA遗传多态性丰富。根据mtDNA序列构建了关中马的NJ分子系统树,发现存在A、C、D、F和G共5个支系,表明关中马是多起源的。
The genetic diversity and phylogenetic evolution of 247 bp mitochondrial DNA （mtDNA） D- loop sequences of 27 Guanzhong horse samples were analyzed by sequencing and bioinformation techniques. The results revealed 9 mitochondrial haplotypes with 19 polymorphic sites, covering 7.69% of the analyzed length of the 247 bp D-loop sequences. Among the polymorphic sites, there were all transitions. The nucleotide diversity （π value） and haplotype diversity （H） estimated were 0. 0219±0. 0076 and 0. 843±0. 041, respectively, showing high mitochondrial genetic diversity in Guanzhong horse breed. The constructed Neighbor - Joining molecular phylogenetic tree based on the 9 mtDNA D loop haplotypes, indicated that lineages A, C, D, F and G were detected which showed multiple maternal origins in Guanzhong horse breed.
出处
《西北农业学报》
CAS
CSCD
北大核心
2008年第6期17-20,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
教育部新世纪优秀人才支持计划(NCET-07-0699)
西北农林科技大学科研专项(07ZR004)
