摘要
目的:观察预先针刺对癫痫持续状态(SE)后神经元损伤的保护作用。方法:建立氯化锂-匹罗卡品大鼠SE模型。神经元尼氏体亚甲蓝特殊染色法观察大鼠海马CA1区神经元尼氏体变化;原位细胞凋亡检测法(TUNEL染色)及电镜观察大脑皮质、海马CA1区神经元凋亡情况。结果:预先针刺组大脑皮质、海马CA1区可见少量棕色TUNEL染色阳性细胞核,较SE组明显减少,尼氏体染色阳性细胞数较SE组明显增多。结论:预先针刺可以减轻SE后神经元损伤,抑制神经元凋亡。
Objective:To observe the protective effect of pro-acupuncture on neuron injury after epilepticism.Methods:Models with epilepticism were made by ip KCl,atropine methobromide and pilocarpine.Neuro Nissl bodies changes in rats' hippocampal CA1 region was observed by methylene blue staining method.Neuro apoptosis in rats' cerebral cortex and hippocampal CA1 region were determined by Tunel staining and electromicroscopy.Results:The positive cells number of methylene blue staining in pro-acupuncture group rats was significantly reduced,that was less than that in the model group.The positive nucleus number of Tunel staining in cerebral cortex and hippocampal CA1 region of pro-acupuncture group rats was decreased obviuosly,that was less than that in the model group.Conclusion:Pro-acupuncture can abate the neuron injury after epilepticism and inhibit apoptosis of neuron.
出处
《中国中医药科技》
CAS
2008年第6期406-407,共2页
Chinese Journal of Traditional Medical Science and Technology
