氯胺酮对发育早期小鼠学习记忆及脑源性神经营养因子表达的影响

Effects on ketamine on learning and memory behavior and BDNF expression in immature mice

目的观察氯胺酮对发育早期小鼠学习记忆能力及脑源性神经营养因子(BDNF)表达的影响。方法取14日龄昆明小鼠50只随机均分为五组:腹腔注射氯胺酮25mg/kg(K1组)、50mg/kg(K2组)、100mg/kg(K3组);生理盐水组(N组),腹腔注射等容生理盐水,每日1次,连续7d;正常对照组(C组)不注射任何药物。在实验第8、9天用跳台法测定小鼠的学习记忆能力,测定结束后取海马测定BDNF的表达。结果与C组比较,K1组学习潜伏期延长、错误次数增加(P<0.05),N组错误次数增加(P<0.01),BDNF的表达减少(P<0.05)。结论反复腹腔注射氯胺酮有可能损害发育早期小鼠的学习记忆能力,BDNF表达的减少有可能是其介导因素。

Objective To investigate the effects of ketamine on the learning and memory behavior and brain derived neurotrophic factor（BDNF） expression in immature mice. Methods Fifty immature mice aged 14 d were randomly divided into 5 groups of ip. ketamine 25 mg/kg（group K1）, 50mg/kg（group K2）, 100 rng/kg（group K3） normal saline（group N）,once a day for 7 days ;rod group C （given nothing as the control）. The learning and memory behavior testing was performed on the 8th and 9th day, after which the expression of BDNF in the hippocarnpuses was detected using imnmnohistochemica and Western blotting method. Results The learning and memory ability of group N and K1 declined compared with that of group C （P〈0.05）. So did the expression of BDNF （P〈0.05）. Conclusion Chronic stress might The learning and memory performance may be mice, hich might be meadiated by a decrease