两种构建大鼠膀胱癌模型方法的比较研究

The comparative study in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU

目的:通过N-正丁基-N-(4-羟基-丁基)亚硝胺[N-butly-N-(4-hydroxybutyl)nitrosamine,BBN](BBN)喂饲和MNU膀胱灌注方法构建大鼠膀胱癌模型,比较两种实验方法的优缺点。方法:BBN诱癌组大鼠使用0.05%BBN溶液连续喂养六周,后改用2%枸橼酸钠溶液作后续喂养22周,第28周为诱导的终点。MNU诱癌组大鼠行MNU膀胱灌注,每两周灌药1次,每次2mg/只,共4次,第10周为诱导的终点。比较两组大鼠的成瘤率和死亡率。结果:BBN诱癌组30只大鼠喂养28周后存活16只,11只成瘤,死亡率46.7%,存活大鼠成瘤率68.8%;MNU诱癌组30只大鼠第10周存活28只,25只成瘤,死亡率6.7%,存活大鼠成瘤率89.3%。结论:利用MNU行大鼠膀胱灌注的方法构建大鼠膀胱癌模型,具有成瘤时间短、成瘤率高、大鼠死亡率低等特点,优于BBN喂饲大鼠成瘤的方法。

Objective：To compare the advantages and disadvantages in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU respectively. Methods： A total of 30 female SD rats weighing about 180-220 gm were given drinking water contain 0.05% BBN for 6 weeks continuously, then fed 2% natrium citricum solution for 22 weeks, 28 weeks later all rats were killed. Other 30 female SD rats were infused intravesically with 2 mg MNU 4 times, at 2 weeks interval, 10 weeks later all rats were killed, then the incidence of tumor growth and mortality of two groups were compared. Results：BBN treatment： 28 weeks later 16 rats were alive and 11 of them had bladder tumor. MNU treatment： 10 weeks later 28 rats were alive, bladder tumor were detected in 25 rats. Conclusions： Intravescular administration of MNU in rats provided a useful model for experimental studies on bladder tumor, the incidence of tumor growth is higher and the mortality of rats is lower than that of BBN treatment.