大鼠磨牙根尖组织的组织学观察

Histological observation of rat molar root tip tissue

目的:观察大鼠根尖组织的组织结构特点,了解根尖诱导成形术的组织学基础。方法:制备SD大鼠(出生后20d)下颌的石蜡组织切片,通过HE染色、免疫组化染色(抗增殖细胞核抗原、抗角蛋白)和天狼星红染色观察SD大鼠下颌第一磨牙根尖组织。结果:下颌第一磨牙牙根发育约2/3,根尖区组织细胞密集且有较多增殖细胞,胶原纤维规律排列,根尖端的上皮根鞘仍保持完整。结论:发育牙根的根尖组织具有较强的组织修复再生能力,保留生活的根尖组织是根尖诱导成形术成功的关键。

Objective：To observe the construction features of root tip tissue and comprehand the histological fundation of apexification. Method： Mandibula of postnatal-20-days SD rats were made into paraffin tissue sections.The root tip construction were observed by HE staining, immunostaining with anti-proliferating cell nuclear antigen （PCNA）and antikeratin antibody and picrosirius-red staining. Result：At PN20d,2/3 length of tooth root of mandibular first molar developed. At the developing tooth root tip, cells closed-up,including many proliferative cells,collagen fibers regttlaly arranged and HERS kept integrity. Conclusion：The tissue of developing tooth root tip possesses great reparative regeneration capacity. It is key to reserve alive tooth root tip tissue for successful apexification.