摘要
目的:观察去除槟榔碱干预后血管内皮细胞(EC)增殖和分泌一氧化氮(NO)的变化。方法:采用MTT法检测去除槟榔碱干预后的EC存活率,硝酸还原酶法检测EC分泌NO量。结果:30μg/ml~90μg/ml槟榔碱干预EC24h,去除刺激并继续孵育48h,EC的增殖能力可恢复,且分泌NO增多(p<0.05);但120μg/ml槟榔碱干预24h、30μg/ml~120μg/ml槟榔碱干预48h并继续孵育48h,EC的增殖能力难以恢复(p<0.05)。结论:去除低浓度、短时间槟榔碱干预后EC增殖能力的恢复可能与具有自我保护效应的NO分泌量增加有关。
Objective: To study the changes of endothelial cell (EC) proliferation and NO production after stimulated by arecoline.Method: Cell survival rate was detected by MTT assay. The NO level of the areeoline stimulated endothelial cell supernatant (AECS) was measured. Result: The proliferation changes of EC induced by arecoline (30 μg / ml- 90 μg / ml) were reversible for only 24 h following 48 h of recovery, but the survival rate of EC decreased after exposure to arecoline (120 μg / ml) for 24 h and arecoline (30 μg / ml-120 μg / ml) for 48 h (P〈0.05).The NO production of AECS was increased after exposure to areeoline for only 24 h following 48 h of recovery (P〈0.05).Conclusion: EC can recover and deplete NO after exposure to arecoline during a short-time culture, the depletion of NO may be a mechanism of EC self-protection.
出处
《临床口腔医学杂志》
2008年第11期662-664,共3页
Journal of Clinical Stomatology
基金
湖南省科技厅社会发展基金资助(02SSY3052)