槟榔碱对血管内皮细胞增殖和分泌NO的影响

Effects of arecoline on proliferation and levels of NO expression of endothelial cell

目的:观察去除槟榔碱干预后血管内皮细胞(EC)增殖和分泌一氧化氮(NO)的变化。方法:采用MTT法检测去除槟榔碱干预后的EC存活率,硝酸还原酶法检测EC分泌NO量。结果:30μg/ml～90μg/ml槟榔碱干预EC24h,去除刺激并继续孵育48h,EC的增殖能力可恢复,且分泌NO增多(p<0.05);但120μg/ml槟榔碱干预24h、30μg/ml～120μg/ml槟榔碱干预48h并继续孵育48h,EC的增殖能力难以恢复(p<0.05)。结论:去除低浓度、短时间槟榔碱干预后EC增殖能力的恢复可能与具有自我保护效应的NO分泌量增加有关。

Objective： To study the changes of endothelial cell （EC） proliferation and NO production after stimulated by arecoline.Method： Cell survival rate was detected by MTT assay. The NO level of the areeoline stimulated endothelial cell supernatant （AECS） was measured. Result： The proliferation changes of EC induced by arecoline （30 μg / ml- 90 μg / ml） were reversible for only 24 h following 48 h of recovery, but the survival rate of EC decreased after exposure to arecoline （120 μg / ml） for 24 h and arecoline （30 μg / ml-120 μg / ml） for 48 h （P〈0.05）.The NO production of AECS was increased after exposure to areeoline for only 24 h following 48 h of recovery （P〈0.05）.Conclusion： EC can recover and deplete NO after exposure to arecoline during a short-time culture, the depletion of NO may be a mechanism of EC self-protection.