热放疗对舌鳞癌Tca 8113细胞及其耐药细胞株耐药性的影响

Effect of Thermoradiotherapy on multidrug resistance of tongue squamous cell carcinoma cell line Tca 8113

目的:探讨热放疗对舌鳞癌Tca8113细胞和耐药的Tca8113/CBDEA细胞耐药性的影响。方法:实时定量逆转录PCR(RT-PCR)检测热放疗对Tca8113细胞和耐药的Tca8113/CBDEA细胞多药耐药基因1(MDR1)、多药耐药相关蛋白1基因(MRP1)和谷胱甘肽硫-转移酶π基因(GST-π)表达的影响和检测热放疗对细胞内阿霉素浓度的影响。结果:Tca8113/CBDEA和Tca8113细胞热放疗后耐药基因MDR1的表达在4h和24h无明显改变(P>0.05)。MRP1基因的表达在4h和24h组均有明显下降(P<0.05),GST-π基因表达在4h组Tca8113和Tca8113/CBDEA的表达无明显改变(P>0.05),在24h组有明显下降(P<0.05).热放疗以后肿瘤细胞内阿霉素(ADM)浓度有明显上升。结论:热放疗联合应用抑制了放疗造成的耐药基因表达上升,增加了细胞内的药物浓度,热放疗联用不会促使肿瘤细胞产生MDR。

Objective： To study effect of thermoradiotherapy on expressions of MDR genes in tongue squamous cell carcinoma cell line Tca 8113 and its muhidrug resistance （MDR） cell line Tca8113 / CBDEA and intracellular ADM concentration.Method： Cell lines were thermoradiotherapy -treated （42 ℃ for 0.5 h and 2Gy of radiotion）, 4 h and 24 h later real-time quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） detected expressions of MDR relative genes （MRP1, MRP1, GST-π） and HTS 7000 Plus Bio Assay Reader measured intraeellular ADM concentration.Result： Post thermoradiotherapy, detection of real time PCR showed there were not alteration in the expression of MDR1 gene （P〉0.05） .Expressions of MRP1 gene significantly descended at 4 h and 24 h postthermoradiotherapy （P〈0.05）.PCR showed there were not alteration in the expressions of GST-π gene at 4h postthermoradiotherapy in Tca 8113 and Tca 8113 / CBDEA cell line （P〉0.05）, but there was significantly decrease at 24 h （P〈0.05）. Drug tolerance decreased.hyperthermia increased intraeellular drug concentration in Tca 8113 / CBDEA and Tca 8113 （P〈0.01）.Conclusion： thermoradiotherapy enhance chemotherapy effect and suppress the expressions of MDR genes induced by radiation.The combination of hyperthermia and radiotherapy does not induce MDR.