摘要
目的探讨外源性人类真核延伸因子（EEF）1A2基因导入人胰腺癌SW1990细胞株后，细胞侵袭转移能力的改变。方法应用腺病毒载体将EEFlA2基因导入人胰腺癌SW1990细胞中，采用划痕实验、Transwell小室、细胞粘附实验检测转染前后细胞运动、侵袭、转移及粘附能力的改变。结果Ad5／F35-EEF1A2转染后继续培养48h的SW1990细胞，可见预期大小的特异性条带。实验组24h后细胞迁移率为（74．43±2．12）％，与阴性对照组和空白对照组比较差异有统计学意义[（44．08±5．92）％和（48．09±3．54）％，P〈0．05]。实验组48h后穿膜细胞数为（65．42±8．24）个，与阴性对照组和空白对照组相比差异有统计学意义[（20．10±5．82）个和（23．25±5．23）个，P〈0．05]。实验组48h后穿膜细胞数为（61．30士5．68）个，与阴性对照组和空白对照组相比差异有统计学意义[（32．04±3．60）个和（32．33±2．51）个，P〈0．05]，且对Ⅰ型、Ⅱ型、Ⅳ型胶原、纤维结合蛋白、粘蛋白的粘附力增强（P〈0．05）。结论腺病毒介导的EEF1A2高表达能明显增强胰腺癌SW1990细胞的运动、侵袭、转移及粘附能力。提示EEF1A2可能通过改变胰腺癌细胞的生物学特性影响胰腺癌的发生发展。
Objective To observe the change of invasion and migration of the pancreatic carcinoma cell line SW1990 transfeeted with EEFIA2 gene. Methods Pancreatic carcinoma cell line SW1990 was transfected with EEF1A2 by recombinant adenovirus vector. The alteration of motility, invasion and adhesion property of SW1990 was evaluated by wound healing assay, transwell With or without Matrigel basement membrane and adhesion assay. Results Wound healing assay revealed that EEF1A2 enhanced cell motility and transwell assay with Matrigel indicated that the average numbers of transwell cells with EEF1A2 was increased from 23.25± 5.23 to 65.42 ± 8.24 （P〈0.05）. The adhesive rate was substantially increased in EEF1A2 transfeeted SW1990 cells compared with control cells. Conclusions EEF1A2 gene can promote the migration,invasion and adhesion ability of pancreatic cancer cell in vitro. It is indicated that EEF1A2 may involve in the development of human pancreatic cancer by influencing cell biological characteristics.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2008年第11期751-754,共4页
Chinese Journal of Digestion
基金
国家自然科学基金资助项目（30670941）
