Clara细胞10-KDa蛋白在小鼠细菌性慢性鼻-鼻窦炎模型中的表达

Expression of Clara cell 10-KDa protein in murine bacterial chronic rhinosinusitis model

目的:观察Clara细胞10-KDa蛋白(CC10)在小鼠细菌性慢性鼻-鼻窦炎(BCRS)模型中的表达。方法:C57BL/6J小鼠用鼻腔阻塞加肺炎链球菌接种的方法建立BCRS模型。用RT-PCR、免疫组织化学、组织病理染色及图像分析方法检测小鼠鼻窦黏膜组织CC10 mRNA及蛋白水平表达、CC10阳性细胞数及鼻窦黏膜形态学参数。结果:BCRS模型组小鼠鼻窦黏膜固有层内多型核中性粒细胞(PMN)数、上皮下胶原纤维沉积、上皮厚度及杯状细胞数均较假手术对照组增多(均P<0.01);BCRS组小鼠鼻窦黏膜上皮CC10阳性细胞数、CC10 mRNA及蛋白表达均较假手术对照组减少(均P<0.01)。CC10阳性细胞数分别与黏膜固有层PMN数、固有层胶原纤维沉积、上皮杯状细胞数及上皮厚度呈显著负相关,相关系数分别为-0.734、-0.776、-0.841和-0.805,均P<0.01;CC10平均灰度值分别与黏膜固有层PMN数、固有层胶原纤维沉积、上皮杯状细胞数及上皮厚度呈显著正相关,相关系数分别为0.771、0.802、0.887和0.855,均P<0.01。结论:小鼠BCRS模型鼻窦黏膜中CC10表达下调。CC10作为一种内源性负调控蛋白可能参与慢性鼻-鼻窦炎的发病过程。

Objective：To investigate the expression of Clara cell 10-KDa protein （CC10） in sinonasal mucosa of murine bacterial chronic rhinosinusitis （BCRS） model. Method：A murine BCRS model was established by Strep- tococcus pneumoniae inoculation plus Merocel ostiomeatal obstruction. After 12 week＇s intervention, histological changes of sinonasal mucosa in BCRS model were examined by hematoxylin and eosin stain, periodic acid-schiff stain, and Masson-Trichrome stain. The mRNA and protein expression of CC10 in sinonasal mucosa were deter mined by reverse transcription polymerase chain reaction and immunohistochemistry methods. The number of CC10 positive cells in sinonasal epithelium was also counted. Result： In BCRS model group, polymorphonuclear neutrophils （PMN）, subepithelial collagen deposition, goblet cells, and epithelial thickness were significantly increased, compared with control group （P〈0.01）. However, CC10 positive cells, CC10 mRNA and protein ex- pression in sinonasal mucosa of BCRS model group were significantly decreased, compared with control group （P〈0.01）. Moreover, the number of CC10 positive cells was significantly negatively correlated with PMN （r=0.734, P〈0. 01）, subepithelial collagen deposition （r=-0. 776, P〈0. 01）, epithelial goblet cells （r= -0. 841, P〈0.01）, and epithelial thickness （r=-0. 805, P%0.01）, respectively. CC10 average grayscale value was significantly positively correlated with PMN （r=0. 771, P%0.01）, subepithelial collagen deposition （r= 0. 802, P〈0.01）, epithelial goblet cells （r=0. 887, P〈0.01）, and epithelial thickness （r=0. 855, P〈0.01）, respectively. Conclusion： The expression of CC10 is downregulated in sinonasal mucosa in BCRS model. As an important endogenous modulin, CC10 might play a crucial role in the pathogenesy of chronic rhinosinusitis.