乙酰肝素酶在鼻咽癌组织中的表达及临床意义

The expression and potentially clinical significance of heparanase in nasopharyngeal carcinoma

目的:探讨乙酰肝素酶(HPA)在鼻咽癌组织中的表达及临床意义。方法:运用免疫组织化学技术检测HPA在70例鼻咽癌和10例鼻咽正常组织中的表达情况,并结合鼻咽癌临床分期、颈淋巴结转移率、复发及转移率、5年生存率等临床病理特征进行分析。结果:鼻咽正常组织中无HPA阳性表达,鼻咽癌组织中HPA阳性表达率为52.9%(37/70)。鼻咽癌临床各期的HPA阳性表达率分别为:Ⅰ期30.0%(6/20),Ⅱ期45.8%(11/24),Ⅲ期70.6%(12/17),Ⅳ期88.9%(8/9)。有、无淋巴结转移组HPA阳性表达率分别为67.4%(31/46)和25.0%(6/24)。HPA阳性、阴性表达者的复发及转移率分别为48.6%(18/37)和15.2%(5/33),5年生存率分别为24.3%(9/37)和78.8%(26/33)。HPA阳性表达与鼻咽癌临床分期、颈淋巴结转移率、复发及转移率、5年生存率等临床病理特征有关(P<0.01)。结论:HPA表达与鼻咽癌的侵袭、转移及预后有关,为抗鼻咽癌治疗提供一个新的可能靶点。

Objective：To investigate the expression of heparanase in nasopharyngeal carcinoma and the relationship between the expression of it and clinically pathological features of nasopharyngeal carcinoma. Method：The expression of heparanase protein in 70 cases of nasopharyngeal carcinomas and 10 cases of normal nasopharyngeal tissues was detected by immunohistochemical staining. The date of expression combined clinical features, which included clinical stage, cervical lymph node metastasis rate, the rate of metastasis and recurrence, combination of, the 5--year survival rate, and other analysis, was analyzed. Result： The positive rate of heparanase protein in can- cerous tissues was 52.9% （37/70）, while it was 0% in normal nasopharyngeal tissues. The positive rates of heparanase protein in patients were 30.0% （6/20） in stage Ⅰ ,45.80% （11/24） in stage Ⅱ , 70.6% （12/17） in stage Ⅲ ,88.9% （8/9） in stage IV respectively. Heparanase positive tumors were associated with a higher inci- dence of lymph node metastasis （67.4%, 31/46） than heparanase negative ones （25.0%, 6/24）. The rate of distant metastasis and regional recurrence in the heparanase positive group was 48.6% （18/37）, but only 15.2% （5/ 33） in the heparanase negative group. The cumulative survival of patients in the heparanase negative group at 5 years was 78.8% （26/33）, but only 24.3% （9/37）in the heparanase positive group. The clinical stage of disease, lymph node metastasis, the rate of distant metastasis and regional recurrence of nasopharyngeal carcinoma were correlated with positive expression of heparanase protein. Conclusion.. The expression of HPA was associated with invasion and metastasis and prognosis of nasopharyngeal cancer, and it may be a new target for the anti--treat- ment of nasopharyngeal cancer. （P〈0.01）, and heparanase expression level inversely correlated with the patient survival （P〈0.01）. Conclusion： Heparanase may play important roles in the invasive infiltration, metastasis, and prognosis in nasopharyngeal carcinoma, clearly indicating that heparanase is a possible target for anticancer drug development.