消瘀化痰方对非酒精性脂肪肝大鼠肝脏解偶联蛋白2 mRNA表达的影响

Effect of the recipe of dispersing blood stasis and eliminating sputum on the expressions of uncoupling protein-2 mRNA in the liver of nonalcoholic fatty liver disease rats

目的观察消瘀化痰方对非酒精性脂肪肝(NAFLD)大鼠肝脏解偶联蛋白2 mRNA(UCP2 mRNA)表达的影响,探讨其防治NAFLD的部分作用机制。方法采用喂饲高脂饲料的方法复制NAFLD大鼠模型,实验分为正常对照组、模型组、东宝肝泰对照组和消瘀化痰方高、低剂量组。提取肝脏总RNA,运用半定量RT-PCR技术观察各组大鼠肝脏UCP2 mRNA的表达情况,同时测定各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)和肝组织匀浆TC、TG的含量,并做病理组织切片。结果模型组大鼠UCP2 mRNA的表达增强,血脂和肝脏脂质含量明显升高,肝脏呈明显脂肪变性。经药物治疗后,各治疗组大鼠肝脏UCP2 mRNA的表达减弱,血脂和肝脏脂质含量显著降低,肝脂变程度明显减轻。结论消瘀化痰方通过调控NAFLD大鼠肝脏UCP2 mRNA的适度表达,可能是其治疗NAFLD的分子机制之一。

Objective To observe the effect of recipe of dispersing blood stasis and eliminating sputum （Xiao-Yu-Hua-Tan-Fang, XYHTF） on the nonalcoholic fatty liver disease （NAFLD） tissue uncoupling protein （UCP）2 mRNA expression, and to explore the part mechanism of treating NAFLD. Methods The NAFLD model rats established by feeding with high fat forage were divided into normal control, model, high and low dose of XYHTF, Dong-Bao-Gan-Tai （DBGT） groups. Total RNA of liver extracted, and the expression of UCP2 mRNA were analyzed by semi-quantitative RT-PCR method. The contents of total cholesterol （ TC ） , triglyceride （ TG ） , free fatty acid （FFA） in serum and the contents of TC, TG in liver tissue homogenate of each group were detected. The pathological section was made. Resuits The model group had significantly increased expression of liver tissue UCP2 mRNA, and lipid content in blood serum and hepatic tissues, greatly denaturalized liver fat cell. After the intervention of medicine, expression of liver tissue UCP2 mRNA of each treatment group was significantly decreased, and lipid in blood serum and hepatic tissues was significantly decreased, liver fat cell denaturizing was improved. Conclusions XYHTF can control the appropriate expression of liver tissue UCP2 mRNA of rat. It is likely to be one of the important mechanisms for treating NAFLD.