98例宫颈癌人乳头状瘤病毒16型E6基因突变及多态性分析

Gene mutation and polymorphism analysis of human papillomavirus type 16 E6 in 98 cervical cancer samples

目的研究湖北地区宫颈癌患者人乳头状瘤病毒16型（HPV16）E6基因点突变分布及频率，与国内外其他研究人员发现的踊突变进行对比，分析历突变在宫颈癌发生中的意义。方法从宫颈癌患者手术切除标本中提取组织DNA，用HPV16E6特异性引物进行PCR扩增，对扩增的历基因片段进行测序分析。结果在35例宫颈癌组织DNA中有18例发生历基因178位核苷酸的突变，变突频率为51．43％，相应核苷酸改变为Asp—Glu，442位核苷酸有4例发生核苷酸序列的改变，突变频率为11．43％，另有10例发生1～2个核苷酸序列的改变。结论湖北地区98例宫颈癌患者人乳头状瘤病毒历基因存在高频率的178和442位核苷酸突变，一些为新发现的核苷酸序列的突变，这些突变在宫颈癌发生中的作用有待于进一步研究。

Objective To study the mutation of papillomavirus type 16 （HPV16） E6 gene, and to compare with the result of other researchers. Methods DNA was extracted from cervical cancer tissue samples, then HPV16 E6 genes were amplified and sequenced using the HPV16 E6 specific primers from cervical cancer DNA samples. Results HPV16 E6 gene mutations at the position of nt178 were found in 18 case from 35 cervical cancer samples, the mutation rate was 51.43%, resulting in nucleotide change of Asp to Glu. E6 mutations are found at the position of nt442 in 4 cervical samples. E6 mutation was found in 1 to 2 case in other nucleotide. Conclusion High frequency mutations of E6 gene of HPV16 at the position of nt178, nt442 have been found in cervical cancer samples in Hubei, China. The role of E6 gene mutation in cervical cancer development should be studied further.