白塞综合征患者外周血中性粒细胞FcγR ⅡA mRNA的表达及其意义

mRNA expression of Fc gamma R Ⅱ A on neutrophils from patients with Behcet＇s disease

目的探讨白塞综合征患者外周血中性粒细胞FcγR ⅡA mRNA表达及其与血浆髓过氧化物酶（MPO）活性的关系。方法采集25例白塞综合征患者活动期及其中15例患者经治疗后的非活动期静脉血，分离中性粒细胞后，以RT-PCR检测其FcγR ⅡA mRNA的表达。采用分光光度法测定血浆MPO活性（代表中性粒细胞激活）。以20例正常人作对照。结果白塞综合征患者外周血中性粒细胞FcγR ⅡA mRNA的表达在活动期为1．801±0．829，非活动期为0．820±0．625，血浆MPO活性分别为32±5U／L和27±4U／L，两者表达在活动期组均高于非活动期组（P〈0．01），非活动期组与正常人对照组比较，差异均无统计学意义（P〉0．05）。白塞综合征患者外周血中性粒细胞FcTRⅡAmRNA的表达水平与血浆MPO活性呈正相关（r=0．39，P〈0．01）。结论白塞综合征活动期外周血中性粒细胞FcTRⅡAmRNA表达升高。推测中性粒细胞的FcγR ⅡA可能与白塞综合征中性粒细胞的激活有关。

Objective To investigate the mRNA expression of Fc gamma R Ⅱ A （FcγR Ⅱ A A） on polymorphonuclear neutrophils （PMN） from patients with Behcet＇s disease （BD）. Methods Twenty-five patients with active BD and 20 healthy human controls were included in this study. Blood samples were obtained from all patients with active BD before treatment, from 15 patients with inactive BD after treatment and from healthy controls. PMN were isolated. The FcγR Ⅱ A mRNA expression on PMNs was detected by RT-PCR, and plasma myeloperoxidase （MPO） activity which represented neutrophil activation, was measured spectrophotometrically. Results The relative expression level of FcγR Ⅱ A A on PMN and plasma MPO activity were 1.801 ± 0.829 and 32 ±5 U/L, respectively, in patients with active BD, 0.820 ± 0.625 and 27 ± 4 U/L, respectively, in those with inactive BD, and 0.745 ± 0.931 and 29 ± 5 U/L, respectively, in normal controls; the differences were significant in the two parameters between the patients with active and inactive BD （both P 〈 0.01 ）, while no statistical difference was observed between inactive patients and normal human controls （P 〉 0.05）. There was a positive correlation between the expression level of FcγR Ⅱ A A on PMN and plasma MPO activity in patients with BD （r = 0.39, P 〈 0.01 ）. Conclusions The mRNA expression of FcγR Ⅱ A A on neutrophils is up-regulated in patients with active BD. It is likely that FcγR Ⅱ A A is involved in the activation of neutrophils in BD.