依匹斯汀等抗组胺药物抗炎作用的研究

Comparative study on the inhibitory effect of several antihistamines on the release of inflammatory mediators from lymphoeytes

目的观察依匹斯汀等几种临床常用抗组胺药物对卵蛋白致敏小鼠脾淋巴细胞表达炎症因子的影响。方法小鼠腹腔注射卵蛋白构建致敏动物模型，分离小鼠脾淋巴细胞，以依匹斯汀等几种抗组胺药物进行阻断处理。用酶联免疫吸附法（EusA）检测培养细胞上清液中白三烯B4（LTB4）、白介素4（IL-4）、白介素5（IL-5）的水平。结果依匹斯汀具有明显的抑制致敏淋巴细胞释放LTB4、IL-4、IL-5的作用，且具有剂量依赖性。在相同作用浓度下，依匹斯汀组致敏淋巴细胞释放的LTB4浓度显著低于依巴斯汀组和西替利嗪组；IL-4浓度显著低于西替利嗪组，与依巴斯汀组相当；IL-5的浓度显著低于西替利嗪、依巴斯汀组。结论依匹斯汀具有较强的抗炎症作用。

Objective Toobserve the effect of several antihistamines, including epinastine, on the release of leukotriene B4 （LTB4）, interleukin 4 （IL-4） and interleukin 5 （IL-5） from spleen lymphocytes of allergic mice challenged by ovalbumin （OVA）. Methods OVA-sensitized BALB/c mice were used as animal model of allergy. Spleen lymphocytes isolated from these mice were treated with epinastine （0.1, 1.0, 10 umol/L）, ebastine （10 umol/L）, cetirizine （10 umol/L）, and dexamethasone （1.0 umol/L）, respectively, followed by incubation with OVA. Those cells without pretreatment with antihistamines served as the control. One hour after the challenge, enzyme-linked immunosorbent assay （ELISA） was applied to measure the level of LTB4, IL-4 and IL-5 in the cell culture supernates. Results Following the incubation with OVA, the levels of LTB4, IL-4 and IL-5 increased significantly in culture supemates of control spleen lymphocytes. Epinastine significantly inhibited the release of LTB4, IL-4 and IL-5 in a dose-dependent manner. At the concentration of 10 umol/L, the inhibitory effect of epinastine was more potent than that of ebastine and cetirizine for IL-5 and LTB4 release （all P 〈 0.05）; for IL-4, the effect of epinastine was similar to that of ebastine （P 〉 0.05） but more potent than that of cetirizine （P 〈 0.05）. Conclusion Epinastine has a potent anti-inflammatory effect.