摘要
本研究初步探讨了重组鸡γ干扰素(rChIFN-γ)对柔嫩艾美耳球虫(E.tenella)卵囊免疫接种鸡肠道粘膜sIgA+细胞数量的影响。分别给7和14日龄雏鸡予以E.tenella孢子化卵囊免疫接种并同时肌注5000u rChIFN-γ后,于21日龄用同源E.tenella攻虫。免疫组化检测结果表明rChIFN-γ加强免疫组在21日龄时十二指肠、空肠、盲肠和回肠的绒毛sIgA+细胞数量均高于免疫对照组,在28日龄时,均呈现显著性差异(α=0.05)。结果表明rChIFN-γ可提高球虫卵囊免疫雏鸡肠道内的sIgA+细胞数量,增强粘膜免疫水平,具有明显的抗球虫免疫增强效果。
The immuomodulatory effects of recombinant chicken interferon-gamma （rChIFN-γ ） on the numbers of secretory IgA （slgA＋） plasma cell in the intestinal mucosa were investigated in chickens vaccinated with E.tenella oocysts. Chickens were simultaneously injected with 5 000 u rChIFN-γ when vaccinated at 7 and 14 day-old, and challenged with 5 ～ 104 homologous E.tenella sporulated oocysts at 21 day-old. The duodenum, jejunum, ileum and cecum regions of the vaccinated chickens were tested for the numbers of slgA＋ plasma cell using immunohistochemical methods. The results showed that numbers of slgA＋ plasma cells lining the intestinal mucosa of broilers received rChIFN-γ injection were significantly higher than that of control broilers only vaccinated with E.tenella occysts （ α =0.05）. Therefore rChIFN-γ had the ability to stimulate the secretion of slgA against the E. tenella in the intestine of immune chickens, and could serve as an effective adjuvant for anticoccodial immunity.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2008年第12期959-963,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家“十五”攻关项目(2002BA514A-17)
