当归提取液对豚鼠心室肌细胞钠、钙离子通道的影响

The Effect of Extractant of Angelica Sinensis Diels on Sodium Current and L Type Callium Current in Isolated Guinea Pig Ventricular Myocytes

应用膜片钳全细胞记录技术观察当归提取液（ＥＡＳ）对酶解分离的单个豚鼠心室肌细胞膜钠通道电流（ＩＮａ）、Ｌ型钙通道电流（ＩＣａ－Ｌ）的影响。当ＥＡＳ为１３２，１１６和１８时，使ＩＮａ峰值（ＩＮａｍａｘ）从６．９８±１．３２ｎＡ分别降至５．７７±１．３３，５．２８±１．１５和３．８２±１．４５ｎＡ（ｎ＝６，Ｐ＜０．０１）；使ＩＣａ－Ｌ的峰值（ＩＣａ－Ｌｍａｘ）从１００５．０±１９６．５ｐＡ分别降至８８０．１±１０５．８，５９７．０±１１０．５和３６４．２±９８．３ｐＡ（ｎ＝６，Ｐ＜０．０１）。ＥＡＳ使ＩＮａ、ＩＣａ－Ｌ的电流－电压曲线上移，但均不改变其激活、峰值和反转电位。以上结果表明ＥＡＳ对ＩＮａ和ＩＣａ－Ｌ具浓度依赖性阻滞作用，这可能是ＥＡＳ具有某些药理效应的离子基础。

Whole cell patch clamp technique was used to investigate the effect of extractant of Angelica Sinensis Diels (EAS) on sodium current (I Na ) and L type callium current (I Ca L ) in enzyme isolated guinea pig ventricular myocytes.EAS at 1∶32,1∶16,1∶8 decreased peak I Na (I Na max ) from 6 98±1 32 nA to 5 77±1 33 nA,5 28±1 15 nA and 3 82±1 45 nA( n =6, P <0.01);decreased peak I Ca L (I Ca L max ) from 1 005 0±196 5 pA to 880 1±105 8 pA,597 0±110 5 pA and 364 2±98 3 pA( n =6, P <0.01).EAS shift up both the I V curve of I Na and that of I Ca L while their active,peak and reverse potentials didnt change.It reveals that EAS blocks both I Na and I Ca L in a concentration dependent manner,which may contribute to some of its pharmacological effect.