支气管哮喘患者治疗前后血清IL-2、IL-8和TNF—α水平的变化及临床意义

Changes of Levels of Interleukin - 2, Interleukin - 8 and Tumor Necrotic Factor- α in Serum During Acute and Stable Phases of Bronchial Asthma and the Clinical Significance

目的：探讨支气管哮喘患者血清白细胞介素-2（IL-2）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF—α）水平的动态变化和检测的临床意义。方法：对30例患者经中药治疗前后及26名正常对照者，采用酶联免疫吸附试验（ELISA）检测血清中上述三种细胞因子的含量。结果：血清IL-2水平在急性发作期患者为（10．2±3．2）ng／ml，明显低于正常对照组（24．8±11．5）ng／ml，P〈0．01，缓解期增至（20．9±5．4）ng／ml，与正常对照组差异无显著性（P〉0．05）；血清IL-8水平在急性发作期患者为（1085±503）ng／ml，明显高于正常对照组（189±71）ng／ml，P〈0．01，缓解期降至（212±115）ng／ml，与正常对照组差异无显著性（P〉0．05）；哮喘发作期TNF—α水平（207．34±31．29））ng／ml，明显高于对照组[（163．48±32．17）mg／ml，（P〈0．01）]，哮喘缓解期TNF-α水平（201．65±33．08）ng／ml高于对照组（P〈0．05），发作期TNF—α水平高于缓解期，但差异无显著性（P〉0．05）。结论：检测血清中IL-2、IL-8和TNF—α7水平变化是判断支气管哮喘病情变化的有益指标。

Objective： To investigate dynamic changes of interleukin - 2 （IL - 2） and interlenkin- 8 （IL - 8） and tumor necrotic factor- α （TNF- α） levels in serum of patients with bronchial and their clinical significance.Methods： The serum IL- 2 and IL - 8 and TNF - α levels were determined in 30 patients during acute phase and after disappearance of symptoms and 26 normal controls by enzymelinked immunosorbent assay （ELISA） .Results： The serum IL-2 level in patients during acute phase was （10.2 ±3.2） ng/ml, it was significantly lower than that of the control group （24.8± 11.5） ng/ml, p〈0.01, and it increased to （20.9 ± 5.4） ng/ml in the stable phase, there was no significant difference between the stable phase and the control group （p〉0.05） . The serum IL-8 level in patients during acute phase was （1085 ± 506） ng/ml, it was significantly higher than that of the control group [ （189 ± 71） ng/ml, p〈0.01], and it reduced to （212 ± 115） ng/ml in the stable phase, no significant difference between the stable phase and the control group was found （p〉0.05） . The serum TNF- α level in patients during acute phase was （207.34 ± 31.29） ng/ml, it was significantly higher than of the control group [ （163.48 ± 32.17） ng/ml, p〈 0.01 ], and the serum TNF - α level in the stable phase was （201.65 ± 32.17） ng/ml, it was significantly higher than in the control group （p〈0.05）, no significant difference between the stable phase and the acute phase was found （p〉0.05） .Conclusion： There is some clinical value to determine the dynamic changes of IL- 2 and IL- 8 levels in patient with bronchial asthma.