摘要
目的探讨脓毒症患者外周血CD^+4CD^+25调节性T细胞水平及其与疾病严重程度的相关性。方法选取脓毒症患者36例,依据诊断标准分为脓毒症组10例、严重脓毒症组15例和脓毒性休克组11例,以健康志愿者5例作为对照组。于人ICU时抽取外周血,分离淋巴细胞,以藻红蛋白(PE)标记的抗人CD4和异硫氰酸荧光素(FTTC)标记的抗人CD25单克隆抗体标记细胞,流式细胞仪检测CD^+4CD^+25调节性T细胞含量。并结合各组不同时间点的急性生理学及慢性健康状况(APACHE)Ⅱ评分,分析CD^+4CD^+25调节性T细胞与APACHEⅡ评分的相关性。结果脓毒症组、严重脓毒症组和脓毒性休克组外周血CD^+4CD^+25调节性T细胞含量[分别为(10.31±2.32)%、(14.27±3.33)%、(15.32±3.98)%]均较对照组[(5.48±0.98)%]显著增高(P〈0.05或〈0.01)。各组CD^+4CD^+25调节性T细胞水平与APACHEII评分呈明显正相关,相关系数分别为0.829(P=0.032)、0.868(P=0.021)、0.913(P=0.009),总相关系数为0.903(P=0.013)。结论脓毒症患者外周血CD^+4CD^+25调节性T细胞明显升高,且与疾病严重程度相关。
Objective To evaluate the correlation between the level of CD^+4CD^+25 regulatory T cells in peripheral blood and disease severity in patients with sepsis. Methods Thirty-six septic patients and 5 healthy controls were enrolled. Septic patients were divided into sepsis group (n=10), severe sepsis group (n=15) and septic shock group (n=11). The lymphocyte was seperated from peripheral blood and marked by PE-CD4 and FITC-CD25 monoclonal antibody, the level of CD^+4CD^+25 regulatory T cells was detected by flow cytometry, and the clinical data of APACHE Ⅱ scores of septic patients was considered in 24 hours. The correlation between level of CD^+4+CD^+25 regulatory T cells in peripheral blood and APACHE Ⅱ scores in septic patients was analyzed. Results Compared with the healthy controls [(5.48±0.98)%], the level of CD^+4CD^+25 regulatory T cells in sepsis group (10.31±2.32) %, severe sepsis group (14.27±3.33)%, septic shock group (15.32±3.98) % had a significant increase (P 〈 0.05 or 〈 0.01 ). The log value of regulatory T cells in each group correlated positively with the APACHE Ⅱ scores (r = 0.829, P = 0.032; r = 0.868, P = 0.021; r = 0.913, P = 0.009), and the total coefficient of correlation was 0.903 (P = 0.013). Conclusion The level of CD^+4CD^+25 regulatory T cells in peripheral blood in septic patients has an abnormal increase, and their levels are related with the severity of disease.
出处
《中国医师进修杂志(内科版)》
2008年第12期16-18,共3页
Chinese Journal of Postgraduates of Medicine