槐耳清膏对人肺腺癌细胞H1299化疗敏感性的影响

Impact of Polysaccharide of Trametes Robiniophila Murr (PS-T) on the Chemosensitivity in Human Lung Adenocarcionoma Cells H1299

[目的]探讨槐耳清膏对p53基因表达缺失的人肺癌细胞系H1299(p53-null)体外生长化疗敏感性的影响。[方法]应用MTT法、流式细胞仪及克隆形成试验,检测槐耳清膏对H1299生长的影响及对化疗药物(DDP和ADM)敏感性的变化。[结果]槐耳清膏能使H1299细胞体外生长速度明显减慢,其抑制生长的作用具有剂量依赖性。MTT实验提示槐耳清膏使顺铂和阿霉素的IC50值分别减少了4倍和45倍,槐耳清膏(1.0g/L)联合原本没有明显抑制和杀伤作用的化疗药物(1.25μmol/L的顺铂或0.25μmol/L的阿霉素)使H1299细胞生长明显受到抑制。流式细胞术显示槐耳清膏使顺铂诱导的细胞凋亡率从12.8%升高到33.5%(P<0.01),阿霉素诱导的细胞凋亡率从22.7%升高到51.0%(P<0.01)。克隆形成实验显示槐耳清膏能明显降低化疗后细胞的克隆形成数(P<0.01),对顺铂和阿霉素的化疗增效倍数分别为1.9和1.6倍。[结论]槐耳清膏可以抑制人肺腺癌细胞H1299体外生长并提高其对阿霉素、顺铂等化疗药的敏感性,且这种作用不依赖于p53基因。

[Purpose ] To investigate the effect of PS-T on growth in vitro and chemosensitivity of H1299 cells （p53-null） of human lung adenocarcinoma. [Methods] The effect on growth of H1299 cells with PS-T was observed by MTT assay. The change of chemosensitivity on cisplatin（DDP）, adriamycin（ADM） was observed by flow cytometry and colony formation assay. [Results] PS-T significantly inhibited growth of H1299 cells in vitro and the antiproliferatitive effect occurred in a dose dependent manner. MTT assay showed that the ICso values for DDP and ADM were reduced to approximate 4 and 45 fold respectively when PS-T combined with chemotherapeutic agent. Chemotherapeutic drugs （1.25μmol/L DDP or 0.25μmol/L ADM）without inhibition essentially suppressed growth of H1299 cells markedly when they combined with PS-T （1.0g/L）. The DDP-induced apoptosis rate increased from 12.8% to 33.5%（P〈0.01）and the ADM-induced apoptosis rate increased from 22.7% to 51.0% （P〈0.01）when they combined with PS-T by flow cytometry. In colony-formation assays, the colony number acted by chemotherapeutic agents significantly decreased when they combined with PS-T（P〈0.01 ）. The sensitive enhancement ratios were 1.9 and 1.6 for DDP and ADM, respectively. [Conclusion] PS-T is capable of inhibiting the growth in vitro of human lung adenocarcinoma cells H1299 and enhancing its sensitivity to chemotherapeutic agents（DDP or ADM）. This role is not depend on p53 gene.