化疗对乳腺癌患者外周血Th1和Th2类细胞因子基因表达的影响

Impact of Chemotherapy on Peripheral Th1 and Th2 Cytokines Gene Expression in Patients with Breast Cancer

[目的]探讨化疗对乳腺癌患者Th1和Th2类细胞因子的基因表达的影响。[方法]采用逆转录聚合酶链反应(RT-PCR)技术,检测乳腺癌患者化疗前后外周血单个核细胞(PBMC)Th1和Th2类细胞因子mRNA表达水平的变化。[结果]乳腺癌组IL-2mRNA(4)、IL-12mRNA(5)阳性表达均较正常对照组(10,14)及良性乳腺肿瘤组(11,16)明显减少(P<0.05);IL-6mRNA(19)、IL-10mRNA(16)较正常对照组及良性乳腺肿瘤组(8,5)显著增加(P<0.05);正常对照组及良性乳腺肿瘤组Th1和Th2类细胞因子mRNA表达无显著性差异(P>0.05)。乳腺癌化疗3个周期后,IL-6mRNA(8/36)和IL-10mRNA(6/38)阳性表达均较化疗前(19/25,16/28)显著降低(P<0.05);IL-2mRNA(14/30)和IL-12mRNA(15/29)阳性表达均较化疗前(4/40,5/39)显著升高(P<0.05)。[结论]化疗可使早期乳腺癌患者Th2类细胞因子的强势表达向Th1类逆转,改善患者的免疫状况。

[Purpose ] To explore the impact of chemotherapy on peripheral Th1 and Th2 cytokines gene expression in patients with breast cancer. [Method] The gene expression of Th1 and Th2 cytokines before and after chemotherapy in breast cancer was detected by reverse transcription polymerase chain reaction （RT-PCR）. [Results] The positive expressions of IL-2 mRNA（4） and IL-12 mRNA（5） of breast cancer patient（breast cancer group） were lower than those of normal control group（10, 14） and benign breast neoplasm group（11,16）（P〈0.05）.The levels of IL-6 mRNA （19） and IL-10 mRNA（16） of breast cancer group were higher than those of normal control group （7, 5） and benign breast neoplasm group（8,5）（P〈0.05）. The positive expressions of IL-6 mRNA（8/ 36） and IL-10 mRNA（6/38） of breast cancer significantly decreased after chemotherapy than those before chemotherapy（19/25, 16/28） （P〈0.05）. The levels of IL-2 mRNA（14/30） and IL-12 mRNA （15/29） of breast cancer significantly increased after chemotherapy than those before chemotherapy （4/40,5/39） （P〈0.05）. [Conclusion] Chemotherapy can reverse the high expression of Th2 eytokines to Thl cytokines in patient with early stage breast cancer resulted in improving the immunity state.