摘要
目的探讨SOCSs基因和JAKs基因在急性髓系白血病（AML）患者中的表达情况。方法RT-PCR方法检测AML患者和正常对照骨髓SOCS1～7、JAK1～3和TYK 2mRNA的表达。结果①AML患者SOCS1、4、5、7的表达明显低于缓解组和正常对照组（P〈0．01），SOCS3、6表达水平较缓解组和正常对照组高（P〈0．01），SOCS2在各组无明显差异；AML患者JAK2、JAK3、TYK2 mRNA平均表达水平较缓解组和正常对照组明显增高（P〈0．05）。初治AML患者JAKl mRNA表达水平较正常对照组略增高，但差异无统计学意义（P〉0．05），复发AML患者JAKl mRNA表达水平较正常对照组明显增高（P〈0．05）。结论AML患者的SOCS1、4、5、7基因表达缺失或降低，JAK家族基因表达明显增高，提示二者可能共同参与髓系白血病的发生。
Objective To investigate the expression of suppressor of cytokine signaling genes （ SOCSs ） and JAKs mRNA in the acute myloid leukemia（AML） patients. Methods The expression of SOCSs and JAKs mRNA as well as TYK2 in AML patients and healthy adults as normal contrals （ NC ） was measured with RT-PCR. Results The expression of SOCS 1,4,5 and 7 in AML patients was lower than those in normal control and AML with remission （ P 〈 0.01 ） , but the expression of SOCS 3 and 6 was higher than those in normal control and remission AML（ P 〈 0.01 ） , however there was no significant difference in SOC2 between groups. The expressions of JAK2, JAK3 and TYK2 in AML were significantly higher than those in patients with remission and normal control （ P 〈 0.05 ）. The expression of JAK1 mRNA in relapsed AML was higher than that in normal control group （ P 〈 0.05 ） , but the latter has no statistical significance between beginning treatment and normal group（P 〉 0.05）. Conclusion The deletion and degradion of SOCS 1,4,5 and 7 present in AML patients and JAKs expression is significantly increased, suggesting that both of them may co-participate in the pathogenesis of AML.
出处
《中国综合临床》
北大核心
2008年第12期1196-1199,共4页
Clinical Medicine of China
基金
河北省自然科学基金项目（C2005000744）
