NF-κB、Survivin与Ki-67在肝癌组织中的表达及其意义

Expressions and significance of NF-κB,Survivin and Ki-67 in hepatocellular carcinoma

目的检测肝组织中Survivin、NF-κB与Ki-67的表达情况,分析其临床病理学意义。方法应用免疫组化SP法检测44例肝细胞癌(HCC)组织、25例癌旁相应组织、9例正常肝组织中Survivin、NF-κB、Ki-67蛋白的表达情况。结果NF-κB蛋白在HCC组织、癌旁组织中的阳性表达率分别为81.82%、28.00%,9例正常肝组织未见表达,3组比较差异有统计学意义(P<0.01);HCC组织学分级Ⅲ、Ⅳ级患者NF-κB表达率显著高于Ⅰ、Ⅱ级者(P<0.05);HCC组织中Survivin蛋白阳性表达率为68.18%,癌旁组织、正常肝组织中均无表达;3组比较差异有统计学意义(P<0.01);Survivin表达与脉管内瘤栓(P<0.05)、组织学分级(P<0.05)显著相关。Ki-67蛋白在HCC组织、癌旁组织中的阳性表达率分别为54.55%、12.00%,9例正常肝组织未见表达,3组比较差异有统计学意义(P<0.01);在HCC组织中组织学分级Ⅲ、Ⅳ级患者Ki-67表达率高于Ⅰ、Ⅱ级者(P<0.05),有脉管内瘤栓患者高于无脉管内瘤栓者(P<0.05)。NF-κB、Survivin与Ki-67的表达均呈显著正相关(P<0.05)。结论联合检测NF-κB、Survivin与Ki-67的表达对于预测肝细胞癌的恶性潜能、判断预后和为肝癌治疗提供新的靶点有一定的临床价值。

Objective To investigate the expressions of apoptotic inhibitor Survivin, nuclear factor-κB（NF-κB） and Ki-67 protein in tissues of hepatocellular carcinoma, and to evaluate their related clinicopathological features of patients with HCC. Methods The expressions of Survivin, NF-κB and Ki-67 protein were examed using immunohisto chemical method in HCC tissues of 44 patients, para-eancerous tissues of 25 patients with HCC , and 9 cases of normal liver. Results The positive rate of NF-κB expression was 81.82% in HCC tissues, 28.00% in para-cancerous tissues, and none in normal liver tissues（ P 〈 0. 01）. NF-κxpressions in grade Ⅲ and Ⅳ were higher than those of grade Ⅰ and Ⅱ in accordance＇ with histological differentiation in HCC （ P 〈 0. 05）. The positive rate of Survivin expression was 68.18% in HCC, while no expression was found in control groups. The expression of Survivin in HCC was significantly correlated to the vein invasion （ P 〈 0.05） and histological differentiation （ P 〈 0.05）. The positive rate of Ki-67 expression in HCC tissues was 54.55%, compared with 12.00% in para-cancerous tissues, and none in normal liver tissues （ P 〈 0. 01） ; The expressions of Ki-67 in grade Ⅲ and Ⅳ were higher than those of grade Ⅰ and Ⅱ in accordance with histological differentiation （ P 〈 0.05）, and their expression were also higher in the cases with vein invasion than those without vein invasion; The expressions of NF-κB , Survivin and Ki-67 were positively correlated with each other（ P 〈 0.05）. Conclusion The current study suggests that detecting the expressions of NF-κB , Survivin and Ki-67 in HCC tissues has a clinical value in predicting the potential malignancy of HCC, evaluating the prognosis and providing the new target for the treatment of HCC.