蛋氨酸铜对西门塔尔牛日粮养分消化代谢及血液指标的影响

Effect of copper methionine on nutrient metabolism and serum physico-chemical parameters in Simmental steer

选用16头平均体重420 kg、年龄2.5岁的中国西门塔尔牛阉牛,采用随机区组设计分为4组,以混合精料和风干玉米秸秆为基础日粮,以蛋氨酸铜为铜源,在基础日粮中分别添加铜0 mg·kg-1、8 mg·kg-1、16mg·kg-1和24 mg·kg-1,研究蛋氨酸铜对西门塔尔牛营养物质消化代谢和血液指标的影响。结果表明:添加铜处理可显著提高有机物质(OM)、粗蛋白质(CP)、粗脂肪(EE)和无氮浸出物(NFE)消化率(P<0.05)。8 mg·kg-1和16 mg·kg-1处理组显著提高中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)消化率(P<0.05)。蛋氨酸铜补充组显著提高消化氮(DN)、沉积氮(RN)和RN/DN。8～16 mg·kg-1蛋氨酸铜补充组较0 mg·kg-1组显著增加血清甘油三酯(TG)、葡萄糖(GLU)、总胆固醇(TC)、白蛋白(ALB)和总蛋白(TP)含量(P<0.05)。24 mg·kg-1处理组血清尿素氮(SUN)明显低于对照组(P<0.05)。随着蛋氨酸铜添加水平的提高,各组间血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)差异均不显著(P>0.05),血清铜蓝蛋白(Cp)逐渐增加(P<0.05),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)活性显著提高(P<0.05),丙二醛(MDA)呈下降趋势(P<0.05)。16 mg·kg-1和24mg·kg-1处理组血清铜含量显著高于对照和8 mg·kg-1处理组(P<0.05)。根据试验结果,蛋氨酸铜添加量以8～16 mg·kg-1为宜。

Sixteen Simmental steers on corn straw diet were stratified by weight and randomly assigned in a block design experiment,which consisted of 0 mg（control）,8 mg,16 mg or 24 mg of supplemental Cu（as copper methionine） per kg dry matter（DM） diet,to evaluate the effect of different copper methionine levels on nutrient digestibility,nitrogen balance and serum parameter.The results show that the digestibility of organic matter,crude protein,ether extract and nitrogen free extract in supplemented Cu treatments increases significantly（P 〈 0.05）.The digestibility of neutral detergent and acid detergent fiber in groups supplemented with 8 mg and 16 mg Cu per kg DM diet are higher than those of the control（P 〈 0.05）.Digestible nitrogen,retention nitrogen and the utilization of nitrogen in Cu treatments increase significantly（P 〈 0.05）.The concentration of serum triglycerides,glucose,total cholesterol,albumin and total protein increases significantly（P 〈 0.05）.Serum urine nitrogen concentration in groups supplemented with 24 mg Cu per kg DM diet decreases significantly（P 〈 0.05）.The activity of serum glutamate oxaloacetate transaminase,glutamate pyruvate transaminase,alkaline phosphatase,acid phosphatase and lactate dehydrogenase is not significantly affected.The activity of serum ceruloplasmin increases significantly（P 〈 0.05）.The activity of serum SOD and GSH-px as well increases significantly（P 〈 0.05）.Serum maleic dialdehyde concentration decreases significantly（P 〈 0.05）.Serum Cu concentration in groups supplemented 16 mg and 24 mg Cu per kg DM diet significantly increases over those of the control and 8 mg Cu per kg group（P 〈 0.05）.Optimum Cu supplemented dose is 8～16 mg Cu per kg DM diet.