新疆维、汉妇女宫颈癌人类染色体端粒酶基因的研究

The study of human chromosome telomerase gene on cervical cancer between Uigur and Han in Xinjiang

目的:探讨维吾尔族(维族)、汉族妇女宫颈癌在感染HPV高危亚型之后,其致癌机制——人类染色体端粒酶(TERC)基因扩增的特点及差异性。方法:采用荧光原位杂交技术(FISH)检测23例维吾尔族和22例汉族宫颈癌患者的TERC基因扩增的情况。结果:23例维吾尔族宫颈癌患者TERC基因扩增率为86.96%,22例汉族宫颈癌TERC扩增率为90.90%。随着临床分期增高,维吾尔族、汉族宫颈癌患者的TERC基因扩增均无明显增加。维吾尔族、汉族宫颈癌在HPV各亚型感染之间,TERC基因平均扩增倍数差异无统计学意义。维吾尔族宫颈癌总的TERC基因扩增倍数与汉族差异有统计学意义,主要与维吾尔族宫颈癌患者中多重感染的比率高于汉族宫颈癌有关。结论:TERC基因的扩增在维、汉族宫颈癌的发生中均为早期事件。维吾尔族宫颈癌患者中多重HPV高危亚型感染比例较高,导致TERC基因扩增明显,可能是维吾尔族宫颈癌发病率高于汉族的原因之一。

Objective： To approach cervical cancer of Uigur and Han in high-risk subtypes of HPV infection, its carcinogenic mechanism-the characteristics and differences of TERC gene amplification. Methods： Use fluorescence in situ hybridization （FISH） detected the situation of human chromosome telomerase （TERC） gene amplification on 23 Uigur patients and 22 Han patients. Results： The TERC gene amplification rate of 23 cases of Uigur cervical cancer was 86.96% and amplified cervical cancer rate of 22 cases of Han uterine cervical cancer was 90. 90%. With the increases of clinical stage, the TERC gene amplification of Uygur and Han cervical cancer patients had not increased significantly. Among the infections of each HPV subtypes, there were no statistical differences on average multiples of TERC gene amplification. However, there were statistical differences on the total of TERC gene amplification multiples between Uigur and Han cervical cancer. It was mainly related with higher rate of multi-infection in Uigur cervical cancer patients, compared with Han patients. Conclusion： TERC gene amplification was the incidence of early gene on both Uigur and Han cervical cancer. The proportion of HPV multi-infection was higher in Uigur cervical cancer patients, compared with Han patients, and leads to TERC gene amplified obviously. So it might be one of reasons for incidence rate of Uigur cervical cancer was higher than Han.