摘要
目的:对羟基红花黄色素A进行提取工艺改进。方法:采用ZTC-Ⅱ型澄清剂甲、D-4020型大孔吸附树脂和Sephadex LH-20柱层析法,从红花中提取分离羟基红花黄色素A单体,采用高效液相色谱法(HPLC)测定羟基红花黄色素A的含量。结果:采用HPLC法测定羟基红花黄色素A的平均含量为97.3%。结论:此工艺具有成本低、纯度较高的优点,可以作为羟基红花黄色素A的提取工艺使用。
Objective： To establish the technology of extraction of HSYA. Methods： Detected the content of HSYA in Emin Carthamus tinctrius L. by using the HPLC method. Extracted and separated HSYA from Carthamus tinctorius L. by using clarifier, D-4020 macroporous resin column chromatography and Sephadex LH-20 chromatography. Results： The mean purity of HSYA was 97.3%. Conclusion： The technology makes clear that the method was cheap and the purity is higher. This technique is suitable to prepare HSYA.
出处
《新疆医科大学学报》
CAS
2008年第10期1358-1360,共3页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2006137)
关键词
红花
羟基红花黄色素A
提取工艺
HPLC
Carthamus tinctrius L.
hydroxysafflor yellow A
extracted technology
high performance liquid chromatography
