二甲基苯并蒽诱导大鼠原位胰腺癌模型的建立

Establishment of animal model of pancreatic cancer in Rats with DMBA

目的:探讨化学致癌剂二甲基苯并蒽(DMBA)诱导Sprague-Dawley(SD)大鼠原位胰腺癌模型建立的有效性。方法:220只SD大鼠,根据术中二甲基苯并蒽用量的不同,随机分为模型组Ⅰ(6 mg组,80只)和模型组Ⅱ(9 mg组,80只)和对照组(60只)。模型组Ⅰ、Ⅱ于胰体尾部切开胰腺被膜后,分别置入6 mg9、mg的二甲基苯并蒽。对照组仅行胰腺被膜切开。模型组术后7个月内每月处死大鼠10只、对照组平均每月处死7只,观察腹腔及胰腺病变,切取胰腺标本。结果:包埋DMBA4个月后大鼠胰腺开始出现腺癌,胰腺癌模型组Ⅰ和组Ⅱ相比,术后6个月癌发生率为70%(7/10),实验大鼠死亡率低,致癌率较高。结论:采用6 mg剂量的二甲基苯并蒽(DM-BA)直接置入胰腺被膜下的实质内,可在短期内获得发生率较高的鼠胰腺癌模型。

Objective. To investigate the efficacy of establishment of animal model of pancreatic cancer in rats with dimethylbenzanthracine （DMBA）. Methods. Two hundred and twenty male Sprague-Dawley rats were divided into control group （60） and DMBA groups （160） including model I group of 80 rats with 6 mg DMBA and model Ⅱ group of 80 rats with 9 mg DMBA. DMBA crystals were implanted into the body and tail parts of the pancreas by sharply opening the pancreatic membrane in model I and Ⅱ groups. Only shamly opening the pancreatic membrane and enclosing were performed in control group. All rats were sacrificed each month in the following 7 months; 7 rats were killed per time in control group, and 10 rats in model Ⅰ model Ⅱ groups. All the rats were underwent abdominal exploration and resection of the pancre- as for pathologic examination. Results. The pancreatic adenocarcinoma was observed in the rats of model I and Ⅱ groups in the fourth month. By comparision, cancerigenic rate in the 6rag group was 70% （7/10） and higher than the 9mg group in 6 months, the death rate was lower. Conclusion. Pancreatic ductal adenocarcinoma may be easily induced in rats with 6 mg DMBA implanted into the pancreas.