EGFR基因在肺腺癌中突变的研究

Epidermal growth factor receptor mutation in lung adenocarcinoma

目的:探讨表皮生长因子受体(EGFR)基因酪氨酸激酶域体细胞在肺腺癌患者中突变的相关因素。方法:分析和检测64例肺腺癌石蜡包埋标本EGFR基因突变状况,采用PCR和序列分析技术进行EGFR基因18、19和21外显子突变分析。结果:64例PAC患者中有16例(25%)酪氨酸激酶域存在体细胞突变。其中2例(3.1%)为18外显子,7例(10.93%)为19外显子,7例(10.93%)为21外显子。结论:肺腺癌(PAC)患者肿瘤组织中EGFR基因突变主要集中于19、21外显子突变,女性高于男性。

Objective： To study Somatic mutation of the tyrosine kinase（TK） domain of the epidermal growth factor receptor（EGFR gene） in lung adenocarcinoma patients.Methods： Mutational analysis for EGFR exons18,19,20 and 21 was performed in 64 patients.Results： Somatic mutations in TK domain of EGFR were identified in 16 of the 64（25%） patients,including 2 cases in exon 18（3.12%）,7 cases in exon 19（10.93%） and 7 cases of in exon 21（10.93%）.Conclusion： EGFR gene mutations is concentrate on 19 and 21 hot sport.The mutation rate of women is higher than that of men.