实验性癫痫时大鼠海马内兴奋性氨基酸受体对内阿片肽含量的影响

THE RELATIONSHIP BETWEEN HIPPOCAMPAL EXCITATORY AMINO ACIDS RECEPTORS AND CONTENTS ENDOGENOUS OPIOID PEPTIDES DURING PENICILLIN- NDUCED SEIZURE IN RATE

应用免疫组织化学方法观察了青霉素致痫及海马内微量注射NMDA受体拮抗剂MK801（5－methy1－10，11－dihydro－5H－dibenzo[a，d]cycloheptan－5，10－iminemaleate)和非NMDA受体拮抗剂DNQX（6，7－dinitroquinoxaline－2，3－dione）后，大鼠海马内强啡肽A1~17和亮啡肽的含量变化。结果发现：在青霉素致痫4h后，海马苔样纤维和门区强啡肽A1~17的含量明显减少，海马CA1区．苔样纤维和门区的亮啡肽含量明显增加。在青霉素致痫前分别在海马内微量注射MK801（6μg）和DNQX（4μg），则在癫痫发作减弱同时，海马内强啡肽A1~17含量较单纯青霉素致痫组增加，亮啡肽含量下降。结果提示：青霉素致痫引起大鼠脑内强啡肽A1~17和亮啡肽含量变化，可能和NMDA受体和非NMDA受体的参与有关。

Immunocytochemical technics were used to evaluate the influence of MK80l(NMDA receptorantagonist) and DNQX (non - NMDAA receptor antagonist) ijected into hipocampus on dynorphin A1~17 and lenenkephalin immunoreactivity in hippocampus during penicillin induced seizure. At 4 h after beginning of seizure itwas found that the content of dynorphin A1~17 was dramatically decreased in mossy fiber and hilus, the content oflen- enkephalin was increased in mossy fiber, hilus and CA1 area of hippocampus. When MK80l (6 μg) andDNQX(4 μg) were injected respectively in hippocampus at 20 minutes before administration of penicillin, thedegree of seizure was decreased. At the same time, the content of dynorphin Al^17 in hippocampus was increased,the content of leu- enkephalin was decreased compared with single administration of penicillin. The resultsindicate that the changes of contents of dynorphin Al^17 and len- enkephalin in the brain of rats during epilepticseizure may be related to the action of NMDA and non- NMDA receptors.