巴曲酶对大鼠大脑缺血及缺血再灌注ET_1 基因表达的影响(英文)

EFFECT OF BATROXOBIN ON ENDOTHELIN-1 GENE EXPRESSION IN ISCHEMIA AND ISCHEMIC REPERFUSION RAT BRAINS

本实验采用大鼠急性脑缺血及缺血再灌注模型，研究巴曲酶对脑缺血及脑缺血再灌注时内皮素（ET1）基因表达的影响。用中大脑动脉（MCA）线检法大鼠模型，共12只，分为缺血组及缺血再灌注组（每组各6只），每组又分为巴曲酶组及盐水组（对照组）。缺血组在缺血后24h，再灌注组则在缺血1．5h及再灌注24h后用原位杂交，并采用IBHS图像分析系统研究ET1基因表达。发现巴曲酶组或对照组手术侧大脑皮质及尾壳核ET1mRNA表达均显著高于对侧（非手术侧）。但是巴曲酶组手术侧的ET1mRNA表达显著低于对照组。结果提示，巴曲酸可使缺血及缺血再灌注ET1基因表达下调。这可能是巴曲酶对缺血再灌注的脑保护作用机理之一。

The present study was aimed to study the association of cerebral ischemia and ischemic reperfusion with endothelin-1 (ET-1 ) gene exression in rat brains as well as the effect of batroxobin on the ET-1gene expression during cerebral ischemia and ischemic reperfusion. Twelve male SD rats were randomly divided into ischemic group(n = 6) and ischemic reperfsion group(n = 6). Each group was subdivided into Batroxobintreated and saline-treated groups. The focal cerebral ischemia and reperfusion models were made with threadembolism of middle cerebral artery. After 24h ischemia and 1. sh ischemia followed by 24h reperfusion, ET--1 geneexpressions were investigated with in situ hybridization and the results were analyzed with IBAS 2000 ImageAnalysis System. It was found that the levels of ET-1 mRNA of cerebral cortex and caudate--putamen were markedlyincreased both in 24h ischemia and ischemic reperfusion groups (P < 0. 01, P < 0. 05, respectively). Inbatroxobin-treated rats, although the ET-1 gene expressions of ischemia and ischemic reperfusion sides were alsoincreased as compared with contralateral control sides, they were significantly lower than batroxobin--non treatedischemia and ischemic reperfusion sides (P< 0. 05, and P < 0. 01 respectively). Batroxobin--induced down--regulation of ET--1 gene expression in cercbral cortex and caudat6--putamen during ischemia and ischemicreperfusion may be one of the protective mechanisms of batroxobin on cerebral ischemia and reperfusion.