摘要
目的:探讨RNA干扰(RNAinterference,RNAi)沉默促肝细胞再生磷酸酶1(phosphatase of regenerating liv-er cell-1,PRL-1)基因对肺癌细胞侵袭力的影响。方法:应用PRL-1基因小干扰RNA(small interfering RNA,siRNA)转染处理肺癌A549细胞后,分别采用实时定量RT-PCR和蛋白质印迹检测PRL-1基因mRNA和蛋白水平,分别采用软琼脂集落培养试验和Boyden小室模型试验检测癌细胞的锚着不依赖性增殖和侵袭能力。结果:与对照组比较,siR-NA转染组PRL-1 mRNA和蛋白水平明显下调,且呈浓度和时间依赖性(P<0.05,P<0.05)。体外试验发现,PRL-1siRNA可有效抑制肺癌细胞集落生长和侵袭能力,且与浓度相关(P<0.05,P<0.05)。结论:PRL-1基因在肺癌侵袭中起着重要的作用,采用PRL-1 siRNA转染可抑制肺癌细胞侵袭。
Objective: To study the effects and mechanism of phosphatase of regenerating liver cell-1 ( PRL-1 ) small interfering RNA(siRNA) on invasion of human lung cancer cell. Methods: After lung cancer cell line A549 were transfected by PRL-1 siRNA, the mRNA and protein of PRL-1 were determined by real time RT-PCR and western blot assay,respectively. The anchorage-independent growth was exmined by clon formation in soft agar, and invasion ability was evaluated by boyden chamber model. Results: The siRNA could down-regulate the level of mRNA and protein of PRL-1 in a dose- and time- dependent manner. Sup- pression of of PRL-1 expression can inhibit invasion ability and anchorage-independent growth in dose-dependent manners( P 〈 0.05, P 〈 0.05 ). Conclusion: PRL-1 gene might play an important role in development of human lung cancer, and down-regulation by PRL-1 siRNA could inhibit invasion of human lung cancer cell.
出处
《江苏大学学报(医学版)》
CAS
2008年第6期480-483,共4页
Journal of Jiangsu University:Medicine Edition
基金
中国博士后科学基金资助项目(2003033547)
关键词
肺肿瘤
促肝细胞再生磷酸酶-1
侵袭
RNA干扰
小干扰RNA
lung carcinoma
phosphatase of regenerating liver cell-l
invasion
RNA interference
small interfering RNA