HPLC法测定速尿止泻胶囊中盐酸小檗碱的含量

Determination of berberine hydrochloride in Suniaozhixie Capsules by HPLC

目的建立速尿止泻胶囊中盐酸小檗碱的含量测定方法。方法采用HPLC法,Kromasil C18柱(250mm×4.6mm,5μm),流动相为0.033mol·L-1磷酸二氢钾溶液-乙腈(60∶40),流速为0.8mL·min-1,检测波长345nm。结果本法盐酸小檗碱浓度在13.8～276μg·mL-1线性关系良好(r=0.9999),平均回收率为99.4%,RSD=1.1%(n=5)。结论本法简便易行,结果准确,重现性好,可用于速尿止泻胶囊的质量控制。

OBJECTIVE To establish the determination of berberine hydrochloride of Sunia0zhixie Capsules. METHODS The HPLC with Kromasil C18 column（250mm× 4.6mm, 5μm）. The mobile phase was 0. 033mol · L^-1 potassium phosphate dibasic soluteion-acetonitride（60 ： 40）. Flow rate was 0.8mL·min^-1 and detection wavelength was 345nm. RESULTS The method had a good linear range of 13.8-276μg·mL^-1（ r =0. 9999）. The average recovery was 99.4%（ RSD = 1.1%, n = 5） . CONCLUSION The method was simple,rapid,accurate and reliable,and can be used to control quality of Suniaozhixie Capsules.