消风止痒颗粒质量标准研究

Quality standard for Xiaofeng Zhiyang Granules

目的建立消风止痒颗粒质量标准。方法采用薄层色谱法对消风止痒颗粒中防风、木通和甘草进行了定性鉴别;用高效液相色谱法测定消风止痒颗粒中升麻苷和5-O-甲基维斯阿米醇苷的含量。结果TLC鉴别方法准确,专属性强;升麻苷在0.152～2.44μg范围内呈良好的线性关系(r=0.9999),升麻苷平均回收率为99.06%,RSD=1.16%(r=5);5-O-甲基维斯阿米醇苷在0.186～2.98μg范围内呈良好的线性关系(r=1.0000),5-O-甲基维斯阿米醇苷平均回收率为99.65%,RSD=0.40%(n=5)。结论所建立的方法可靠、准确、专属性强,可有效控制消风止痒颗粒的质量。

OBJECTIVE To establish the quality standard for Xiaofeng Zhiyang Granules. METHODS The Radix Saposhnikcviae, Akebia quinata （Thunb.）Decne and Radix Glycyrrhizae were identified by TLC;The content of Prim-O-Glucosyleimifugin was dentermined by HPLC. RESULTS The spots in TLC were quite clear and the linear range of icariin was 0. 152-2.44μg （ r =0. 9999）. The average recovery was 99.06% withRSD 1.16%（ n =5）. The linear range of 4-O-glucopyranosyl-5-O- methylvisamminol was 0. 186-2.98μg （ r =0. 9999）. The average recovery was 99.65% with RSD 0. 40%（ n =5）. CONCLUSION The method is reliable,specific and accurate,and can effectively control the quality of Xiaofeng Zhiyang Granules.