海南长臀鮠人工繁殖与胚胎发育的研究

Studies on artificial propagation and embryonic development of Cranoglanis bouderius multiradiatus

使用LHRH—A2＋DOM＋PG混合催产剂对从海南引进经池塘驯化养殖5冬龄的海南长臀鲍进行催产，并用日产80i光学显微镜观察胚胎发育过程。取得平均催产率88．9％，平均受精率82．3％，平均孵化率68．7％的效果。成熟的卵子为圆球形、淡黄色、粘性卵。在水温24．8～27．0℃条件下，其受精卵经细胞期、囊胚期、原肠期、体节出现期、器官出现期和出膜前期等多个发育时期孵化出膜，所需时间为82h 15min。拍摄了胚胎发育照片25幅。

In this study, Cranoglanis bouderius multiradiatus was introduced from Hainan and domesticated about five years in pond. The mixture of LHRH - A2 ＋ DOM ＋ PG was injected into Cranoglanis bouderius multiradiatus before their embryonic development was observed by Nikon 80i optical microscope. These results were concluded. The average of spawning rate （ induction rate） was 88.9%, and the average fertilization rate and hatchability （hatching rates） were 82.3% and 68.7%, respectively. Their mature eggs were spheroidal, light, yellow and sticky. The cycle of embryonic development included six stages （ cell stage, blastula stage, gastrula stage, somite genesis stage, organ stage and hatching stage） through eighty two hours and fifteen minutes at 24.8 - 27.0 ℃ of water temperature. In the experiment, twenty-five fetation photographs were taken to describe the whole process.