地衣芽孢杆菌和荚膜红假单胞菌对异育银鲫鱼种非特异性免疫机能的影响

Effects of Bacillus lichenifirmis and Rthodopseudcmonas capsulata on the non-specific immune function of Allogynogenetic crucian carp

本试验主要研究芽孢杆菌和光合细菌对异育银鲫鱼种血液白细胞数量、白细胞吞噬活性及血清溶菌酶活性的影响。600尾异育银鲫鱼种,随机分成9个试验组,一个对照组,每组设一平行。试验组在水中添加不同浓度(5×108、5×109、5×1010CFU/m3)的芽孢杆菌和光合细菌及两种菌1∶1的混合制剂,饲养49 d后,测定异育银鲫血液白细胞数目、白细胞的吞噬活性、血清溶菌酶活性等各项免疫指标。试验结果表明:光合细菌、芽孢杆菌及其1∶1的混合制剂均能提高异育银鲫鱼种血液中白细胞的免疫机能,其中混合制剂作用效果最佳,其次芽孢杆菌,效果最差的是光合细菌,且在5×108、5×109、5×1010CFU/m3浓度范围内,微生态制剂的作用效果随着添加量的增加而增加。

The effects of Bacillus sp. and photosynthesis bacteria on white cell number in the blood, phagocytes activities and the serum lysozyme activities in AUogygenetic crucian carp were studied in the experiment. 600 Allogygenetic crucian carps were divided randomly into 9 treated groups and a control with a paralleled team. Different concentrations （5×10^8、5×10^9、5×10^610 cfu/m^3） of Bacillus sp. , photosynthesis bacteria or mixture agent of Bacillus sp. and photosynthesis bacteria （ 1： 1 ） in the water were prepared for treated groups. After 49 days feeding, white cell number, phagocytes activities and serum lysozyme activities in the tested fish were measured. The experimental results showed that Bacillus sp. , the mixture agent and photosynthesis bacteria could strengthen the non-specificity immune function of Allogynogenetic crucian carp. The effect of mixture agent was the best, the second was Bacillus sp. and the last was photosynthesis bacteria.The effect of the microecological agent increases with the rise of its concentration.