ORYZALIN不同处理方法对西瓜四倍体诱变效果的影响

Influence of Different ORYZALIN Treatments on Tetraploid Mutagenic Effect of Watermelon

以不同西瓜二倍体自交纯系为实验材料,以播前除草剂ORYZALIN为诱变剂,比较了不同处理方法(浓度为25,40 mg/L和55 mg/L的ORYZALIN溶液田间滴苗;浓度为125,200 mg/L和275 mg/L的ORYZALIN羊毛脂浸膏涂抹;40 mg/L的ORYZALIN溶液浸种及浸芽处理)以及滴苗处理后不同整枝方式对西瓜四倍体的诱变效果。不同处理方法对四倍体诱变效果的综合比较结果表明:以40 mg/L,6 d滴苗处理和125 mg/L涂抹处理效果最佳,其诱变率分别达到17.39%和36.36%;浸芽处理诱变率最高仅为3.33%,干种子浸种处理未见明显变异。不同材料间的四倍体诱变效果具有一定差异。

The inbred lines of diploid watermelon were used for experiment material. Mutagenic effect of tetraploid were compared by using herbicide ORYZALIN as mutagen in different treatment methods （dropping seedling treated by ORYZALIN at 25 mg/L,.40 mg/L and 55 mg/L;smearing treated by ORYZALIN at 25 mg/L,40 mg/L and 55 mg/L; seed soaking,germinated seeds were immersed into 40 mg/L ORYZA- LIN solution） and different ways of stem training. The results showed that the mutagenic effect of tetraploid were best with 40 mg/L ORYZALIN for 6 d in drop seedling and with 125 mg/L in smearing growing point, the mutation rate was up to 17.39% and 36.36% ;and it was up to 3. 33% the highest mutation rate after treatment with soaking seed. There was no significant variation in the treatment of dry seed with soaking. Mutagenic effect of tetraploid was different in various diploid inbred lines.