CYP2E1遗传型与饮酒习惯对广西肝癌易感性的影响

THE EFFECT OF INTERACTION BETWEEN ALCOHOL DRINKING AND POLYMORPHISMS OF CYTOCHROME P450 2E1 ON THE SUSCEPTIBILITY OF HEPATOCELLULAR CARCINOMA IN GUANGXI REGION

目的:研究细胞色素P450 2E1(CYP2E1)基因多态性与饮酒因素交互作用在广西原发性肝细胞癌(肝癌)发生中的作用。方法:对广西壮族自治区300例肝癌和292例正常对照进行流行病学调查研究,并用PCR-RFLP方法检测CYP2E1基因型。结果:病例组和对照组中变异基因CYP2E1者分别占32.3%和32.9%(P>0.05)。饮酒频度每周≥3次(高频饮酒)且携带野生基因和变异基因CYP2E1者发生肝癌的危险度分别是饮酒频度每周<3次(低频饮酒)且携带野生基因型者的1.299倍和1.803倍。结论:单一易感基因CYP2E1变异基因型与肝癌易感性无关;频繁饮酒可能提高CYP2E1变异基因型携带者患肝癌的危险性。提示对于CYP2E1变异基因型携带者,高频饮酒是一个重要的危险因子。

Objective： To study the relationship between CYP2E1 gene polymorphism and the susceptibility of hepatocellular carcinoma（HCC）. Methods： 300 cases of HCC and 292 controls were genotyped for the CYP2E1 polymorphisms by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Result： The frequencies of CYP2E1 variant genotypes in the cases and controls were 32.3% and 32.9%, respectively. There was no significant difference of CYP2E1 genotypes distribution between the cases and controls （ P〉0.05）. The risk for liver cancer was 1. 299 times and 1. 803 times higher in alcoholics （≥3 times drinking per week） with CYP2E1c1 and CYP2E1c2 genotype than that in the cases car- rying CYP2E1c1 genotype while drinking less than 3 times per week （ P =0. 286 and 0. 051）, respectively. Conclusion： CYP2E1 genotypes alone render no significant risk for HCC, while frequent alcoholic Consumption together with CYP2E1 variant genotypes may be associated with risk of hepatocarcinogenesis, suggesting a gene-environment interaction in increasing risk for HCC among Guangxi residents.