小麦株高的QTL分析

QTL mapping for plant height of wheat using a F_(2:3) population

【目的】研究控制小麦株高的数量性状位点（QTL）。【方法】利用SSR和AFLP分子标记构建连锁图谱，在3种不同试验环境（2003～2004年北京、2004～2005年北京和河南安阳）下分析百农64×京双16组合的218个F2：3株系群体的株高。【结果】构建了由158个分子标记（100个SSR标记和58个AFLP标记）位点组成的遗传连锁图谱，覆盖了除1D连锁群外小麦全基因组的3114cM；检测到3个控制株高的QTL，分别位于2B、4D和6A染色体上，贡献率分别为7．3％～11．5％，7．4％～12．9％和5．7％～11．3％。【结论】3个株高QTI。位点在不同环境下表现稳定，其紧密连锁的分子标记可用于矮秆、半矮秆小麦的标记辅助育种。

[Objective] The research was done in order to study QTLs controlling plant height in wheat. [Method] A population with 218 F2,3 lines derived from the cross between Bainong 64 and Jing- shuang 16 was detected using SSR and AFLP markers in three environments,i, e. Beijing（2003--2004）, Beijing and Anyang （2004--2005）. [Result] The results showed that a linkage map with 100 SSR and 58 AFLP markers,covering 3 114 cM of the wheat genome except chromosome 1D, was constructed. Three QTLs for plant height were found on chromosomes 2B, 4D and 6A, which accounted for 7.3 %- 11.5 %, 7.4%--12.9% and 5.7%-11.3% of phenotypic variance respectively. [Conclusion] These QTLs showed stable effects across three environments, and thus their closely linked molecular markers could be useful in marker-assisted-selection programs for breeding dwarf and semi-dwarf wheat cuhivars.