大鼠创伤性脑损伤后血中神经元特异性烯醇化酶变化的实验研究

The dynamic changes of plasma neuron-specific enolase following traumatic brain injury in rats

目的研究大鼠液压冲击性脑损伤后血中神经元特异性烯醇化酶(NSE)的动态变化,探讨NSE在创伤性脑损伤中的作用。方法建立液压损伤模型,观察成年性Wistar大鼠168只:实验组(n=126)和对照组(n=42)。实验组分为3个亚组,每组各42只大鼠。实验组用液压冲击致伤,造成大鼠轻、中、重三型脑损伤模型,运用酶联免疫吸附试验(ELSIA)技术测定NSE在0.5、2、6、12、24、48h和72h(n=6)血浆的变化。结果对照组大鼠血浆NSE浓度在较低的水平,实验组大鼠NSE血浆浓度明显高于相应时间的对照组水平,实验组在0.5、12h大鼠血浆NSE浓度随损伤程度增加而增加,重度损伤组血浆NSE浓度0.5h即开始明显增高,达到峰值,12h达到第2个峰值;中度损伤组血浆NSE浓度2h达到峰值,24h达到第2个峰值;轻度损伤组血浆NSE浓度2h达到峰值,48h达到第2个峰值。损伤程度越重,血浆NSE浓度高峰出现越早。结论NSE可以作为创伤性脑损伤早期标志,可能参与了创伤性脑损伤的病理生理过程。

Objective To investigate the plasma changes of neuron-specific enolase （NSE） in traumatic brain injury （TBI） caused by fluid percussion rats and the role of NSE in TBI. Methods 168 healthy adult Wistar rats were randomized into the injury （n=126） and control groups （n=42）. To create TBI models, the rats assigned to injury group were rendered different degree of TBI （mild, moderate and severe） caused by fluid percussion （n=42 for each degree of injury）. The plasma levels of NSE were determined with ELISA at 0.5, 2, 6, 12, 24, 48 and 72 h from TBI in every 6 rats from the groups. Results At each time spot, higher level of NSE was found in TBI models compared with the low values in controls. At 0.5 h and 12 h, higher plasma levels of NSE were associated with more severe degree of TBI. The peak and resurgence of NSE were observed at 0.5 h and 12 h in severe TBI, 2 h and 24 h in moderate TBI, and 2 h and 48 h in mild TBI. More severe injury was linked to earlier NSE peaking. Conclusion NSE may be involved in the pathophysiological process of TBI and may be used as an early biomarker.