血铅对细胞免疫黏附功能影响的研究

Effect of blood lead level on fresh blood quick native immune reaction to cancer cells

目的评估血铅对儿童细胞免疫黏附（TRR、TLR、TGR）的影响，探讨血铅对细胞免疫黏附的影响程度以及血铅临界值与细胞免疫黏附的关系。方法根据血铅值将儿童分为3组：≥0．48μmol／L组26例，0．24—0．48μmol／L组40例，〈0．24μmol／L组54例。①采用石墨炉原子吸收光谱法测定血铅水平。细胞免疫黏附测定采用新鲜血免疫黏附肿瘤细胞花环实验。②血铅与细胞免疫黏附肿瘤细胞的关系采用直线相关分析。结果高铅组与低铅组比较细胞免疫黏附率差异显著。铅分界值越高免疫黏附率改变越明显。①≥0．48μmol／L组儿童TRR和TLR表达明显低于〈0．24μmol／L组（t=3．48，2．32，P〈0．01）。②血铅值≥0．48μmol/L时血铅水平与TRR、TLR表达呈显著负相关（r=-0．703，-0．606，P〈0．01）。结论铅对小儿细胞免疫黏附功能有不良影响，两者存在相关关系。

Objective To evaluate the significance of blood level, as an indicator for environmental lead, and fresh blood quick native immune reaction to cancer cells as an effect indicator, and to determinate the correlation between the content of blood lead and quick native immune reaction to cancer. Methods A cohort of children were allocated to three groups based on their blood lead levels （designed as group 1, ≥0.48 μmol/L, n=26; group 2, 0.24～0.48 μmol/L, n=40; and group 5, 〈0.24 μmol/L, n=54）. The blood lead levels were measured with graphite furnace atomic absorption spectroscopy and fresh blood quick native immune reaction to cancer cells was assessed using rosette formation test. Linear correlation analysis was used to study the relationship between blood lead level and expression of cancer cells adding in fresh anti-coagulate. Results Data from all 120 children was used for analysis. Expression of TRR and TLR were lower in Group 1 than in Group 3 （t=3.48, 2.32, P〈0.01）. Above a level of t〉0.48 μmol/L, blood lead showed significant inverse correlation with TRR and TLR （r=-0.705, -0.606, P〈0.01）. Conclusion High blood lead level may have adverse effect on native immune reaction in children.