奶牛隐性乳房炎的检测及病原菌的分离鉴定与药敏试验

Detection of Cow Recessive Mastitis and Isolation Identification and Drug Sensitivity Test of Pathogen

试验对内蒙古包头市某奶牛场饲养的451头生产奶牛的1728个乳区进行检测、病原菌分离鉴定及药敏试验。结果显示,患隐性乳房炎的奶牛252头,阳性率为55.9%;有效乳区1728个,阳性乳区480个,乳区阳性率为27.8%。从检测为隐性乳房炎阳性的乳区乳汁中分别分离到金黄色葡萄球菌、链球菌、大肠杆菌、棒状杆菌、沙门氏菌和表皮葡萄球菌,检出菌中传染性致病菌金黄色葡萄球菌和链球菌的检出率高。从药物敏感性试验结果看,头孢唑啉和沙星类药物除了沙门氏菌以外对其他菌均高度敏感,常用药物呈现出不同程度的耐受性;狼毒中草药对链球菌有一定的敏感性,对葡萄球菌和大肠杆菌的抑菌效果不好,对棒状杆菌和沙门氏菌没有抑菌作用;乳炎金针为中西药合剂,对6种致病菌抑菌效果都比较好。

1728 mammary regions from 451 production cows reared in a cattle farm of Baotou City in Inner Mongolia were detected, and the isolation identification and drug sensitivity test of pathogen were made in the experiment. The result showed that there are 252 cows suffered from recessive mastitis and the positive rate was 55.9 %; there were 1728 effective mammary regions, 480 positive mammary region and the positive rate of mammary region was 27.8 %. Staphylococcus aureus, streptococcus, Escherichia coli, corynebacterium, salmonella and Staphylococcus epidermidis were isolated from the milk of detected positive mammary region of rec, essive mastitis, resp. The detection rate of infectious bacteria of S. aureus and Streptococcus in the detected bacteria were higher. From the result of drug sensitivity test, cefazolin and quinolone drug had high sensitive to other bacteria except salmonella, and the common drugs showed tolerance to a different degree; the euphorbia Chinese herbal medicine had certain sensitivity to streptococcus, poor antibacterial effect on E. coli and staphylococcus, no antibacterial effect on corynebacterium and salmonella; Ruyanjinzhen was the mixture of Chinese traditional and Western medicine, which had better antibac, terial effect on the 6 pathogenic bacteria.