斑贞1号联合氟尿嘧啶对胃癌细胞SGC-7901/ADR的细胞毒作用

Cytotoxicity effect of combination of Banzhen-1 and fluorouracil on multidrug resistance of gastric carcinoma cells SGC-7901/ADR

目的探讨斑贞1号联合氟尿嘧啶(5-FU)对胃癌SGC-7901/ADR细胞的细胞毒作用。方法以SGC-7901/ADR细胞为靶细胞,分别设5-FU组、斑贞1号组、斑贞1号+5-FU组,采用四甲基偶氮唑盐(MTT)法观察各药物组的细胞毒作用,光镜下动态拍照并用流式细胞仪测定各药物组细胞周期的变化。结果斑贞1号联合5-FU半数抑制浓度(IC50)分别由单独用药时的(7.85±0.03)mg/L(、13.00±0.13)mg/L降至(4.75±0.04)mg/L(P<0.01)。对SGC-7901/ADR细胞毒作用斑贞1号+5-FU>斑贞1号>5-FU。光镜下斑贞1号+5-FU组细胞与单独用药组相比,细胞生长明显受到抑制,癌细胞体积变小,胞体全面皱缩。流式细胞仪检测斑贞1号+5-FU组SGC-7901/ADR细胞明显阻滞在G0/G1期,进入S期细胞减少,抑制癌细胞DNA的合成(P<0.05)。结论斑贞1号联合5-FU较单独用药对SGC-7901/ADR细胞具有明显的细胞毒作用。

Objective To investigate the eytotoxicity effect of combination of Banzhen-1 and fluorouracil（5-FU） on muhidrug resistance of gastric carcinoma cells SGC-7901/ADR. Methods SGC-7901/ADR was considered as target cell and 5-FU, Banzhen-1 and Banzhen-1 ＋ 5-FU were considered as experiment groups. Methyl thiazolyl tetrazolium （MTT） assay was used to investigate half inhibition 50% inhibiting concentration（IC50） of different medicine groups in vitro,which was observed by light microscope. Flow cytometer（FCM） was employed to investigate the cell cycle. Results IC50 of Banzhen-1 and 5-FU were reduced from （7.85±0.03） mg/L and （13.00±0.13） mg/L to （4.75± 0.04） mg/L （ P 〈0.01）. The cytotoxieity effect against SGC-7901/ADR cells was Banzhen-1＋5-FU〉Banzhen-1〉5- FU. The light microscope revealed that the size of cells exposed to Banzhen-1＋ 5-FU became smaller and the cells shrinked than other groups. FCM revealed that cells cycle was stopped at presynthetic phase and ambiguous phase-G0/ G1 phase and the cells that entered the period of DNA synthesis were reduced, so mitosis of cells were inhibited（ P 〈 0.05） with the effect of Banzhen-1＋5-FU. Conclusion Combination of Banzhen-1 and 5-FU has stronger eytotoxicity effect against SGC-7901/ADR.