HPLC测定小青龙口服液中的苦杏仁苷

Content determination of amygdalin in Xiaoqinglong oral solution by HPLC

目的建立测定小青龙口服液中苦杏仁苷的方法。方法采用HPLC法,流动相为水-甲醇-乙腈(75:20:5),检测波长为210nm。结果小青龙口服液中苦杏仁苷的平均回收率为99.02%,RSD=1.37%,含量为1.3575mg·g-1。结论所建方法操作简便、准确,可作为小青龙口服液中苦杏仁苷的含量控制方法。

OBJECTIVE To determine the amygdalin in Xiaoqinglong oral solution. METHODS HPLC was adopted and the mobile phase was water - methanol - acetonitrile（70 ： 25 ： 5）. The detection wavelength was 210 nm. RESULTS The average recovery of amygdalin in Xiaoqinglong oral solution was 99.02% with RSD of 1.37% and the content was 1. 3575 mg.g^-1. CONCLUSION Besides simplicity, the method yields accurate and reproducible results ,which can therefore be used in the quality control of amygdalin content in Xiaoqinglong oral solution.