胀果甘草细胞的悬浮培养

Cell Suspension Culture of Glycyrrhiza inflate

切取胀果甘草(Glycyrrhiza inflate)无菌苗的子叶、下胚轴和根段外植体进行愈伤组织的诱导和悬浮细胞培养,建立了胀果甘草细胞悬浮培养体系,测定了悬浮培养细胞的生长曲线,并且研究了接种量、条件培养和激素组合对胀果甘草悬浮细胞生长的影响.结果表明子叶来源的愈伤组织最适合作为胀果甘草细胞悬浮培养的起始培养物;悬浮培养的最佳接种量为35 g/L;条件培养基的最佳用量为总培养体积的1/3;最适合胀果甘草悬浮细胞生长的培养基是附加了NAA 1.0 mg/L+KT 0.5 mg/L激素组合的MS培养基.

Different explants from plantlet of Glycyrrhiza inflate were cultured on the MS medium containing different phytohormones to induce callus and suspension cell culture was established when the induced callus were transferred to liquid medium. The cultures were incubated at （25 ± 1） ℃ in the dark with continuing agitation （120 r/min）. On the basis of this, the growth curve of suspension cell of Glycyrrhiza inflate was measured. In this study, the author have investigated the effects of the initial callus amount, conditioned medium （CM） and combination of phytohormone auxin and cytokinin on the growth of suspension cell. The results indicated that the callus induced from cotyledon was the best initial callus for cell suspension culture among the three different explants; the optimal initial c allus amount for cell sus as one third of total volume and the optimal medium was MS ＋ NAA 1.0 mg/L ＋ KT 0.5 mg/L.