新生猪缺氧缺血性脑病脑中Ca^(2+)、CaM、PDE的变化

Changes of Calcium Calmodulin and Phasphodiesterase in the Brain Damage of Newborn Pigs of Hypoxic Ischemic Encephalopathy

通过新生猪缺或缺血性脑损伤模型,检测了脑皮质组织钙、钙调素含量以及磷酸二脂酶活性的变化,探讨在缺氧缺血性脑损伤过程中以上三指标的改变.方法 采用改进的Thompson CaM激活PDE间接测定法检测CaM,反应液中除去工具酶PDE及CaM标准液检测PDE,利用火焰原子吸收分光光度法检测Ca^(2+).结果 脑损伤后单位体积湿重脑组织含钙量变化不明显,钙调素含量及磷酸二脂酶活性分别在损伤后即刻及12小时明显升高.结论 缺氧缺血性脑损伤早期就有钙调素含量的升高,继而引起其靶酶活性升高,造成神经系统的损害.

The changes of calcium (Ca2+), calmodulin (CaM) and phosphodi-esterase (PDE) were tested in the brain damage model of newborn pigs hypoxic ischemic encephalopathy (HIE). Methods PDE indirect determine of improvement Thompson CaM, PDE and CaM were removed in the response solution and atom spectropho-tometry. Results The brain Ca2+ content was not change at unit volume of fresh cerebral tissue,CaM content and PDE activity rose significantly at that time and 12 hours of the brain damage. Conclusion the changes of CaM content and PDE activity were related to the brain damage of HIE.