凋亡相关基因的整合分析及基因表达芯片的制备

Analysis and Preparation of Oligonucletide Microarray of Apoptosis-related Genes

利用IPI和GenBank,收集了与凋亡相关的5333条基因序列,用一定的标准,整合筛选得到1384个凋亡相关的基因.通过亚细胞定位、组织表达差异显著性分析、自然正义/反义RNA对预测、基因簇在pathway上的定位、蛋白质/蛋白质相互作用等方面的分析发现,一些基因只在一个组织里显著差异表达,一些基因存在自然正义/反义RNA对现象,一些基因簇同时位于多条pathway等重要信息.同时,制作了一张凋亡相关基因的寡核苷酸芯片,并且对于一对NAIF1表达质粒转染前后的HeLa样品,通过该芯片的筛选,得到24个差异表达的基因.发现NAIF1过表达诱导的细胞凋亡,伴随了PAX2、PDCD8、PDCD10、DFFA、CASP7等基因表达的显著变化,同时还发现,U58668,该mRNA没有任何基因或蛋白质的注释信息,当camptothecin诱导U937细胞系凋亡时上调,在这里的NAIF1过表达诱导的HeLa细胞系中也上调(上述数据结果见http://gpcrome.cbi.pku.edu.cn:2005/chip).

With some conditions, there are 1 384 genes selected from 5 333 possible apoptosis sequences which were mined from IPI and GenBank. After the analysis of their subcellular location, tissue expression remarkably, natural antisense transcripts predict, gene clusters distributed in pathways, protein protein network, some interested things were mined. Some genes are differentially expressed in a tissue type; Some genes are NATS and some gene clusters are in more than one pathway. Meanwhile, one 40-bp oligonucleotide microarray which includes most apoptosis genes was made. With the microarray and samples of HeLaT-NAIFI and HeLaT-pcDNA3, there were 24 genes differentialy expressed. Perhaps, when the Naif1 was over expressed. PAX2, PDCD8, PACD10, DFFA, CASP7 were also expressed differentially. And there is one mRNA, U58668, neither any gene nor protein information annotation, upregulated during camptothecin-induced apoptosis of U937 cells, also upregulated when the NAIF1 induced HeLaT cell apoptosis（all data is public in http：//gpcrome.cbi.pku.edu.cn：2005/chip）.