酪氨酸激酶受体RON在上皮性卵巢癌中的表达及其意义

Expression of tyrosine kinase receptor RON in epithelial ovarian carcinoma and its clinical significance

目的:研究酪氨酸激酶受体RON在上皮性卵巢癌组织中的表达及其与临床病理学参数的关系。方法:采用逆转录聚合酶链反应和免疫组织化学方法分别检测42例上皮性卵巢癌新鲜组织RON mRNA及其对应32例石蜡组织中RON蛋白的表达。结果:上皮性卵巢癌新鲜组织中RON mRNA阳性表达率为57.14%,对应的上皮性卵巢癌石蜡组织中RON蛋白平均面密度值为(0.0601±0.0284),RON mRNA和蛋白表达均与上皮性卵巢癌组织的临床分期、组织学分级及淋巴结转移有关。结论:RON的过度表达与上皮性卵巢癌的进展、转移密切相关,检测RON的异常对判断肿瘤的临床进展及转移有一定的参考价值,RON可能成为诊断治疗的新靶点。

Objective： To investigate the relation of tyrosine kinase receptor RON expression with clinicopathological parameters, and observe the expression of RON in epithelial ovarian carcinoma （EOC）. Methods： NON mRNA was detected in 42 fresh tumor tissues of EOC using reverse transcription polymerase chain reaction （RT-PCR）. RON protein was examined in 32 corresponding paraffin-embedded tissues by immunohistochemistry. Results： The RON mRNA positive rate in EOC was 57. 14% . The average area density of R（）N protein was 0. 060 1±0. 028 4. The statistical evaluation revealed a close relation among the clinical stage, histologic grade, lymphatic metastasis and the expression of RON mRNA and protein. Conclusion： Excessive expression of RON is closely related with the malignant progression and metastasis of EOC. Detection of RON may be helpful to evaluate the differentiation and tumor progression of EOC. RON may be a new diagnostic and therapeutic targert of EOC.