应激性溃疡对延髓内脏带的影响及雷贝拉唑预处理的作用

Influence of stress ulcer on medullary visceral zone and effects of rabeprazole pretreatment

目的:探讨应激性溃疡与延髓内脏带(MVZ)的关联性,并观测雷贝拉唑预处理的影响。方法:SD大鼠随机分为对照组、应激组和预处理组。延髓冷冻切片后用SABC法检测神经元特异性烯醇化酶(NSE)和S-100蛋白的表达,胃窦部石蜡切片后行H-E染色。ELISA法检测血清NSE和S-100蛋白水平。结果:应激组胃黏膜有局灶性出血和脱落,延髓内脏带的NSE和S-100蛋白阳性表达较对照组显著增多,且NSE和S-100蛋白水平明显升高;预处理组胃黏膜病理改变较应激组明显改善,MVZ的NSE和S-100蛋白阳性表达明显减少,血清NSE和S-100水平较应激组显著降低。对照组胃黏膜无病理性改变,MVZ的NSE和S-100蛋白阳性表达很少,血清NSE和S-100蛋白水平极低。结论:(1)力竭性运动加水浸-束缚应激可导致应激性胃溃疡;(2)应激性溃疡对MVZ的神经元和神经胶质细胞有伤害性影响;(3)雷贝拉唑预处理对MVZ起到了保护性作用,其机制可能与预防应激性胃溃疡的发生有关。

Objective： To explore the influence of stress on medullary visceral zone （MVZ） and inquire the effects of pretreatment by rabeprazole. Methods： Healthy SD rats were randomly divided into three groups： control group, stress group and pretreatment group. The expression of NSE and S-100 protein in frozen section of medullary was detected by immunohistochemistry staining and the pathological changes of antrum were observed by HE staining. The changes of NSE and S-100 protein in serum was measured by Elisa. Results： Compared with the control group, the stress group showed that pathological changes was obvious in gastric mucosa and the expression of NSE and S-100 protein was significantly increased in MVZ. The injury of antrum and MVZ was not remarkable in the pretreatment group. The levels of NSE and S-100 protein in serum increased remarkably in the stress group compared with the control group, and the change in the pretreatment group was not obvious. Conclusion： 1. Compound stimulation could induce stress ulcer successfully. 2, The injury of MVZ was related to stress ulcer. 3. Rabeprazole has highly protective effects on MVZ, of which the possible protective mechanism was closely related to against stress ulcer.